Probing the role of region 2 of Escherichia coli sigma 70 in nucleation and maintenance of the single-stranded DNA bubble in RNA polymerase-promoter open complexes.
作者信息
deHaseth Pieter L, Tsujikawa Laura
机构信息
Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106-4935, USA.
Mol Microbiol. 2009 Jul;73(2):152-64. doi: 10.1111/j.1365-2958.2009.06758.x. Epub 2009 Jun 16.
3
The -11A of promoter DNA and two conserved amino acids in the melting region of sigma70 both directly affect the rate limiting step in formation of the stable RNA polymerase-promoter complex, but they do not necessarily interact.