Tasdemir Deniz, Demirci Betül, Demirci Fatih, Dönmez Ali A, Baser K Hüsnü Can, Rüedi Peter
Institute of Organic Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Z Naturforsch C J Biosci. 2003 Nov-Dec;58(11-12):797-803. doi: 10.1515/znc-2003-11-1208.
Volatile constituents of various solvent extracts (n-hexane, CH2Cl2, H2O) of 15 different organs (leaves, flowers, fruits) of five Rhododendron species (Ericaceae) growing in Turkey were trapped with headspace solid-phase microextraction (HS-SPME) technique and analyzed by GC-MS. A total of 200 compounds were detected and identified from organic extracts, while the water extracts contained only traces of few volatiles. The CH2Cl2 extract of the R. luteum flowers was found to exhibit the most diverse composition: 34 compounds were identified, with benzyl alcohol (16.6%), limonene (14.6%) and p-cymene (8.4%) being the major compounds. The CH2Cl2-solubles of R. x sochadzeae leaves contained only phenyl ethyl alcohol. This study indicated appreciable intra-specific variations in volatile compositions within the genus. Different anatomical parts also showed altered volatile profiles. This is the first application of HS-SPME-GC-MS on the volatiles of Rhododendron species.
采用顶空固相微萃取（HS-SPME）技术捕集了生长在土耳其的5种杜鹃花科杜鹃花属植物的15种不同器官（叶、花、果实）的各种溶剂提取物（正己烷、二氯甲烷、水）中的挥发性成分，并通过气相色谱-质谱联用仪（GC-MS）进行分析。从有机提取物中总共检测并鉴定出200种化合物，而水提取物中仅含有少量挥发性成分的痕量物质。发现淡黄杜鹃花朵的二氯甲烷提取物具有最多样化的成分：鉴定出34种化合物，其中苯甲醇（16.6%）、柠檬烯（14.6%）和对伞花烃（8.4%）为主要化合物。索恰德杜鹃叶片的二氯甲烷可溶物仅含有苯乙醇。该研究表明该属植物挥发性成分存在明显的种内差异。不同的解剖部位也显示出不同的挥发性特征。这是HS-SPME-GC-MS首次应用于杜鹃花属植物的挥发物分析。
