Karasaki Yuji, Kashiwazaki Hiroshi
University of Occupational and Environmental Health, School of Health Sciences, Yahatanishi-ku, Kitakyushu, 807-8555 Japan.
Ann Clin Biochem. 2004 Jan;41(Pt 1):57-60. doi: 10.1258/000456304322664717.
To investigate whether population differences in food and/or lifestyle could affect the distribution frequencies of polymorphism in the gene for beta3-adrenergic receptor (beta3-AR), the frequency of Trp64Arg polymorphism was studied among Bolivian people living in rural areas of high (about 4000 m above sea level) and low (about 300 m above sea level) altitudes.
Genomic DNA samples of Bolivian subjects (n=508) were amplified by polymerase chain reaction (PCR) for part of the beta3-AR gene. The amplified PCR products were digested with restriction enzyme NciI and analysed by agarose gel electrophoresis.
We found no significant difference in the frequency of Arg allele in the beta3-AR gene between 331 native low-altitude Bolivian subjects (18.1%) and 177 native high-altitude Bolivian subjects (17.5%). Body mass index was not associated with Trp64Arg polymorphism among native Bolivian adults. The frequency of this allele in the complete Bolivian population (18%) was lower than that reported in Pima Indians (32%), is comparable to the Japanese (19%) and is higher than several ethnic groups, including Finns (12%) and French (4%).
Our data indicate that the altitude-related lifestyle of a population has had little influence on the frequency of Trp64Arg polymorphism and obesity in Bolivian natives.
为了研究食物和/或生活方式的人群差异是否会影响β3 - 肾上腺素能受体（β3 - AR）基因多态性的分布频率，我们在生活于高海拔（约海拔4000米）和低海拔（约海拔300米）农村地区的玻利维亚人群中研究了Trp64Arg多态性的频率。
对玻利维亚受试者（n = 508）的基因组DNA样本进行聚合酶链反应（PCR）扩增β3 - AR基因的部分片段。扩增的PCR产物用限制性内切酶NciI消化，并用琼脂糖凝胶电泳分析。
我们发现，331名低海拔玻利维亚本地受试者（18.1%）和177名高海拔玻利维亚本地受试者（17.5%）的β3 - AR基因中Arg等位基因频率没有显著差异。玻利维亚成年本地人中，体重指数与Trp64Arg多态性无关。该等位基因在整个玻利维亚人群中的频率（18%）低于皮马印第安人报道的频率（32%），与日本人的频率（19%）相当，高于包括芬兰人（12%）和法国人（4%）在内的几个种族群体。
我们的数据表明，人群中与海拔相关的生活方式对玻利维亚本地人Trp64Arg多态性频率和肥胖影响不大。
