McNealy Tamara L, Trojan Lutz, Knoll Thomas, Alken Peter, Michel Maurice Stephan
Department of Urology, University Hospital, Mannheim, Germany.
Urol Res. 2004 Aug;32(4):255-60. doi: 10.1007/s00240-003-0321-6. Epub 2004 Jan 9.
The use of doxorubicin as a chemotherapeutic agent is hindered by its toxic side effects on the normal cells of the body. The objective of this study was to determine if micelle-delivered doxorubicin could increase the effectiveness of doxorubicin against prostate carcinoma cells. Rat prostate carcinoma cells (MatLu) were cultured under standard conditions. Phosphate-buffered saline (PBS), doxorubicin and/or micelle solution (Pluronic 10500 solution) was added to the cell suspensions and incubated for 3 h. After incubation, cells were washed twice. Analysis consisted of: 1) immediate cell count and 2) proliferation assay at 24 and 144 h. After 24 h, samples with micelle-incorporated doxorubicin had 75% (10% pluronic with 10 microg/ml doxorubicin) and 80% (1% pluronic with 10 microg/ml doxorubicin) cell proliferation results compared with the control group. After 144-h incubation, these same two groups demonstrated cell proliferation results of only 30 and 43% of the control group. The in vitro cytotoxicity of doxorubicin against prostate carcinoma cells was dramatically increased by incorporating the molecule with polymeric micelles.
阿霉素作为一种化疗药物，其对机体正常细胞的毒副作用限制了它的使用。本研究的目的是确定胶束递送的阿霉素是否能提高阿霉素对前列腺癌细胞的疗效。大鼠前列腺癌细胞（MatLu）在标准条件下培养。将磷酸盐缓冲盐水（PBS）、阿霉素和/或胶束溶液（普朗尼克10500溶液）加入细胞悬液中，孵育3小时。孵育后，细胞洗涤两次。分析包括：1）即时细胞计数和2）在24小时和144小时时的增殖测定。24小时后，与对照组相比，含有胶束包裹阿霉素的样品细胞增殖结果分别为75%（10%普朗尼克与10微克/毫升阿霉素）和80%（1%普朗尼克与10微克/毫升阿霉素）。孵育144小时后，这两组细胞增殖结果仅为对照组的30%和43%。通过将阿霉素与聚合物胶束结合，其对前列腺癌细胞的体外细胞毒性显著增加。
