日本儿童幽门螺杆菌与TT病毒感染率

Helicobacter pylori and TT virus prevalence in Japanese children.

作者信息

Kato Seiichi, Okamoto Hiroaki, Nishino Yoshikazu, Oyake Yasuo, Nakazato Yutaka, Okuda Masumi, Fujisawa Takuji, Iinuma Kazuie, Blaser Martin J

机构信息

Department of Pediatrics, Tohoku University School of Medicine, 1-1 Seiryo-machi, Aoba-ku, 980-8574 Sendai, Japan.

出版信息

J Gastroenterol. 2003;38(12):1126-30. doi: 10.1007/s00535-003-1218-4.

DOI:10.1007/s00535-003-1218-4

PMID:14714248

Abstract

BACKGROUND

The major transmission route of Helicobacter pylori, oral-oral or fecal-oral, remains to be established. TT virus (TTV), a recently discovered microbe that is prevalent in healthy persons, is believed to be mainly transmitted by nonparenteral routes. The purpose of this study was to test the hypothesis that these two microorganisms have a common mode of transmission.

METHODS

We investigated the seroprevalence of H. pylori and TTV in a cross-sectional study of 454 healthy Japanese children from birth to age 15 years, living in five different geographic areas. Determination of H. pylori status was based on the presence of specific serum IgG and IgA antibodies, determined using enzyme immunoassays. TTV DNA was detected and the titer was determined using semiquantitative polymerase chain reaction with heminested primers.

RESULTS

The overall prevalences of H. pylori and TTV were 12.2% and 21.6%, respectively. An age-related increase of prevalence was shown for H. pylori ( P < 0.001), but not for TTV ( P = 0.23). Titers of TTV DNA significantly decreased with age (P = 0.02). There were significant geographic differences in TTV prevalence ( P < 0.001), but not in H. pylori seroprevalence (P = 0.33). There was no true correlation between the prevalence of these two organisms (Phi coefficient = -0.02 and P = 0.66).

CONCLUSIONS

Although Japanese children frequently acquire both H. pylori and TTV, especially in early childhood, their acquisition appears to be independent.

摘要

背景

幽门螺杆菌主要的传播途径，即口口传播或粪口传播，仍有待确定。TT病毒（TTV）是一种最近发现的在健康人群中普遍存在的微生物，据信主要通过非肠道外途径传播。本研究的目的是检验这两种微生物具有共同传播方式这一假设。

方法

我们在一项横断面研究中调查了454名年龄从出生到15岁、居住在五个不同地理区域的健康日本儿童的幽门螺杆菌和TTV血清流行率。幽门螺杆菌感染状态的确定基于使用酶免疫测定法检测到的特异性血清IgG和IgA抗体的存在情况。使用半定量聚合酶链反应和套式引物检测TTV DNA并确定其滴度。

结果

幽门螺杆菌和TTV的总体流行率分别为12.2%和21.6%。幽门螺杆菌的流行率显示出与年龄相关的增加（P<0.001），但TTV并非如此（P = 0.23）。TTV DNA滴度随年龄显著下降（P = 0.02）。TTV流行率存在显著的地理差异（P<0.001），但幽门螺杆菌血清流行率无此差异（P = 0.33）。这两种微生物的流行率之间没有真正的相关性（Phi系数 = -0.02，P = 0.66）。