[牛睾丸透明质酸酶的分离与纯化]
[Isolation and purification of bovine testicular hyaluronidase].
作者信息
Barsukov A K, Kozhevnikova O V, Khokhriakova A V
机构信息
Udmurt State University, Izhevsk, 426034 Russia.
出版信息
Prikl Biokhim Mikrobiol. 2003 Nov-Dec;39(6):625-9.
PMID:14714474
Abstract
We developed a single-step method for chromatographic purification of the hyaluronidase extract on Sepharose blue, which produced high yield of the enzyme (95%) with high purity (40). Purification of hyaluronidase allowed us to obtain biological preparations standardized by the protein composition and enzyme activity and useful for basic and applied researches.
摘要
我们开发了一种在琼脂糖蓝上对透明质酸酶提取物进行色谱纯化的单步方法，该方法能以高纯度（40）高产率（95%）获得该酶。透明质酸酶的纯化使我们能够获得按蛋白质组成和酶活性标准化的生物制剂，这些制剂对基础研究和应用研究都很有用。
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