Morita Naokatsu, Hagihara Masao, Kanai Nobuyuki, Hiraga Seigo, Saito Akira, Sakai Hideto, Tsuji Kimiyoshi
Department of Nephrology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Tokai J Exp Clin Med. 2003 Jul;28(2):71-80.
To select congenial pairs between donor-pig and recipient-human for the future xenotransplantation, the levels of xeno-IgM natural antibodies (NAb) were analyzed in healthy subjects and hemodialysis patients by ELISA tests, which target swine-derived crude endothelial cells (P16N) or proteins (thyroglobulin; TG). The total IgM concentration was lower in hemodialysis patients than in healthy subjects, but there was no difference in IgM NAb titer between the two groups. Individuals with non-B blood types (A, O) exibited significantly higher IgM NAb titer compared with those with B blood types (B, AB). A blood type individuals showed higher killing activity against P16N than those with B, AB or O types with a statistical significance. Sera from A blood type, after being absorbed with red blood cells (RBC) from B blood type, decreased their IgM titer against TG to the level of sera from B blood type. Meanwhile, sera from A blood type significantly decreased hemagglutinin titer against B-RBC after passage through a TG-coated affinity column. We conclude that human anti-B-RBC and anti-Pig xeno NAb have certain common binding epitopes, which might be a branched B carbohydrate structure.
为了筛选出适合未来异种移植的供体猪和受体人之间的匹配对，通过酶联免疫吸附测定（ELISA）试验分析了健康受试者和血液透析患者体内针对猪源粗制内皮细胞（P16N）或蛋白质（甲状腺球蛋白；TG）的异种IgM天然抗体（NAb）水平。血液透析患者的总IgM浓度低于健康受试者，但两组之间的IgM NAb滴度没有差异。非B血型（A、O）个体的IgM NAb滴度显著高于B血型（B、AB）个体。A型血个体对P16N的杀伤活性高于B型、AB型或O型血个体，具有统计学意义。A型血血清用B型血红细胞（RBC）吸收后，其针对TG的IgM滴度降至B型血血清水平。同时，A型血血清通过TG包被的亲和柱后，其针对B-RBC的血凝素滴度显著降低。我们得出结论，人类抗B-RBC和抗猪异种NAb具有某些共同的结合表位，可能是一种分支的B碳水化合物结构。
