编码134千道尔顿蛋白的马巴贝斯虫基因的分子克隆及其在酶联免疫吸附测定中的诊断潜力评估。
Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.
作者信息
Tamaki Yoh, Hirata Haruyuki, Takabatake Noriyuki, Bork Sabine, Yokoyama Naoaki, Xuan Xuenan, Fujisaki Kozo, Igarashi Ikuo
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
出版信息
Clin Diagn Lab Immunol. 2004 Jan;11(1):211-5. doi: 10.1128/cdli.11.1.211-215.2004.
Abstract
A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could effectively differentiate B. caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection.
摘要
用感染马巴贝斯虫的马血清对编码134-kDa（BC134）蛋白的马巴贝斯虫基因进行免疫筛选。使用重组BC134蛋白的酶联免疫吸附测定（ELISA）能够有效地区分感染马巴贝斯虫的马血清与感染驽巴贝斯虫或未感染的对照马血清。这些结果表明，重组BC134蛋白是检测马巴贝斯虫感染的一种潜在诊断抗原。
相似文献
1
Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.编码134千道尔顿蛋白的马巴贝斯虫基因的分子克隆及其在酶联免疫吸附测定中的诊断潜力评估。
Clin Diagn Lab Immunol. 2004 Jan;11(1):211-5. doi: 10.1128/cdli.11.1.211-215.2004.
2
Cloning and expression of a 48-kilodalton Babesia caballi merozoite rhoptry protein and potential use of the recombinant antigen in an enzyme-linked immunosorbent assay.48千道尔顿的马巴贝斯虫裂殖子棒状体蛋白的克隆与表达以及重组抗原在酶联免疫吸附测定中的潜在应用
J Clin Microbiol. 1999 Nov;37(11):3475-80. doi: 10.1128/JCM.37.11.3475-3480.1999.
3
Cloning of a truncated Babesia equi gene encoding an 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay.编码82千道尔顿蛋白的截短马巴贝斯虫基因的克隆及其在酶联免疫吸附测定中的潜在用途。
J Clin Microbiol. 2002 Apr;40(4):1470-4. doi: 10.1128/JCM.40.4.1470-1474.2002.
4
Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.重组杆状病毒介导的马巴贝斯虫裂殖子抗原1在昆虫细胞中的表达及其在酶联免疫吸附试验中的诊断潜力评估
J Clin Microbiol. 2001 Feb;39(2):705-9. doi: 10.1128/JCM.39.2.705-709.2001.
5
Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses.用于同时血清学诊断马巴贝斯虫和驽巴贝斯虫感染的免疫层析试验。
Clin Vaccine Immunol. 2006 May;13(5):553-5. doi: 10.1128/CVI.13.5.553-555.2006.
6
Detection of equine antibodies to babesia caballi by recombinant B. caballi rhoptry-associated protein 1 in a competitive-inhibition enzyme-linked immunosorbent assay.在竞争性抑制酶联免疫吸附试验中，用重组马巴贝斯虫动基体相关蛋白1检测马对马巴贝斯虫的抗体。
J Clin Microbiol. 1999 Jul;37(7):2285-90. doi: 10.1128/JCM.37.7.2285-2290.1999.
7
Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis.克隆一种编码对血清学诊断有用的158千道尔顿蛋白的新型马巴贝斯虫基因。
Clin Diagn Lab Immunol. 2005 Feb;12(2):334-8. doi: 10.1128/CDLI.12.2.334-338.2005.
8
Serum antibodies from a subset of horses positive for Babesia caballi by competitive enzyme-linked immunosorbent assay demonstrate a protein recognition pattern that is not consistent with infection.通过竞争性酶联免疫吸附试验检测，部分感染马巴贝斯虫呈阳性的马匹血清抗体显示出与感染不一致的蛋白质识别模式。
Clin Vaccine Immunol. 2013 Nov;20(11):1752-7. doi: 10.1128/CVI.00479-13. Epub 2013 Sep 18.
9
Expression of Babesia equi merozoite antigen-2 by recombinant baculovirus and its use in the ELISA.
Int J Parasitol. 1999 Nov;29(11):1803-8. doi: 10.1016/s0020-7519(99)00146-0.
10
Evaluation of enzyme-linked immunosorbent assays with recombinant antigens for the serodiagnosis of equine Babesia infections.用于马巴贝斯虫感染血清学诊断的重组抗原酶联免疫吸附测定法的评估。
Vet Parasitol. 2006 Aug 31;140(1-2):158-61. doi: 10.1016/j.vetpar.2006.03.013. Epub 2006 Apr 18.
引用本文的文献
1
Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA.通过 ELISA 开发一种有前景的抗原混合物，用于全球检测马巴贝斯虫。
PLoS One. 2023 Apr 14;18(4):e0284535. doi: 10.1371/journal.pone.0284535. eCollection 2023.
2
Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis.克隆一种编码对血清学诊断有用的158千道尔顿蛋白的新型马巴贝斯虫基因。
Clin Diagn Lab Immunol. 2005 Feb;12(2):334-8. doi: 10.1128/CDLI.12.2.334-338.2005.
本文引用的文献
1
Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis.马巴贝斯虫P82蛋白特定抗原区域的鉴定及其在血清学诊断中的潜在应用。
J Clin Microbiol. 2003 Feb;41(2):547-51. doi: 10.1128/JCM.41.2.547-551.2003.
2
Cellular localization of Babesia bovis merozoite rhoptry-associated protein 1 and its erythrocyte-binding activity.牛巴贝斯虫裂殖子棒状体相关蛋白1的细胞定位及其红细胞结合活性。
Infect Immun. 2002 Oct;70(10):5822-6. doi: 10.1128/IAI.70.10.5822-5826.2002.
3
Cloning of a truncated Babesia equi gene encoding an 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay.编码82千道尔顿蛋白的截短马巴贝斯虫基因的克隆及其在酶联免疫吸附测定中的潜在用途。
J Clin Microbiol. 2002 Apr;40(4):1470-4. doi: 10.1128/JCM.40.4.1470-1474.2002.
4
Characterisation of the gene encoding a protective antigen from Babesia microti identified it as eta subunit of chaperonin containing T-complex protein 1.对微小巴贝斯虫保护性抗原编码基因的表征显示，它是包含T复合物蛋白1的伴侣蛋白的η亚基。
Int J Parasitol. 2001 Dec;31(14):1673-9. doi: 10.1016/s0020-7519(01)00278-8.
5
Expression of Babesia equi merozoite antigen 1 in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.重组杆状病毒介导的马巴贝斯虫裂殖子抗原1在昆虫细胞中的表达及其在酶联免疫吸附试验中的诊断潜力评估
J Clin Microbiol. 2001 Feb;39(2):705-9. doi: 10.1128/JCM.39.2.705-709.2001.
6
Molecular characterization of the amplified carboxylesterase gene associated with organophosphorus insecticide resistance in the brown planthopper, Nilaparvata lugens.褐飞虱中与有机磷杀虫剂抗性相关的扩增羧酸酯酶基因的分子特征分析
Insect Mol Biol. 2000 Dec;9(6):647-53. doi: 10.1046/j.1365-2583.2000.00229.x.
7
Cloning, characterization, and expression of a 200-kilodalton diagnostic antigen of Babesia bigemina.双芽巴贝斯虫200千道尔顿诊断抗原的克隆、特性鉴定及表达
J Clin Microbiol. 2000 Jun;38(6):2240-7. doi: 10.1128/JCM.38.6.2240-2247.2000.
8
Detection of Babesia caballi infection by enzyme-linked immunosorbent assay using recombinant 48-kDa merozoite rhoptry protein.
Int J Parasitol. 2000 Apr 24;30(5):633-5. doi: 10.1016/s0020-7519(00)00008-4.
9
Cloning and expression of a 48-kilodalton Babesia caballi merozoite rhoptry protein and potential use of the recombinant antigen in an enzyme-linked immunosorbent assay.48千道尔顿的马巴贝斯虫裂殖子棒状体蛋白的克隆与表达以及重组抗原在酶联免疫吸附测定中的潜在应用
J Clin Microbiol. 1999 Nov;37(11):3475-80. doi: 10.1128/JCM.37.11.3475-3480.1999.
10
Molecular detection of Babesia equi and Babesia caballi in horse blood by PCR amplification of part of the 16S rRNA gene.通过对16S rRNA基因部分片段进行PCR扩增，对马血中的马巴贝斯虫和驽巴贝斯虫进行分子检测。
Vet Parasitol. 1999 Jul;84(1-2):75-83. doi: 10.1016/s0304-4017(99)00049-7.
Zotero 插件