Prates Maura V, Sforça Maurício L, Regis Wiliam C B, Leite José R S A, Silva Luciano P, Pertinhez Thelma A, Araújo Antônio L T, Azevedo Ricardo B, Spisni Alberto, Bloch Carlos
Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília-DF, Brazil.
J Biol Chem. 2004 Mar 26;279(13):13018-26. doi: 10.1074/jbc.M310838200. Epub 2004 Jan 8.
Amphibian skin secretions constitute an important source of molecules for antimicrobial drug research in order to combat the increasing resistance of pathogens to conventional antibiotics. Among the various types of substances secreted by the dermal granular amphibian glands, there is a wide range of peptides and proteins, often displaying potent antimicrobial activities and providing an effective defense system against parasite infection. In the present work, we report the NMR solution structure and the biological activity of a cationic 14-residue amphiphilic alpha-helical polypeptide named Hylaseptin P1 (HSP1), isolated from the skin secretion of the hylid frog Hyla punctata. The peptide antimicrobial activity was verified against Candida albicans, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, whereas no significant lytic effect was detected toward red or white blood cells.
两栖动物的皮肤分泌物是抗微生物药物研究中分子的重要来源，以应对病原体对传统抗生素日益增加的耐药性。在两栖动物皮肤颗粒腺分泌的各类物质中，有多种肽和蛋白质，它们通常具有强大的抗菌活性，并提供针对寄生虫感染的有效防御系统。在本研究中，我们报告了从雨蛙科斑点雨蛙（Hyla punctata）的皮肤分泌物中分离出的一种名为Hylaseptin P1（HSP1）的阳离子14残基两亲性α-螺旋多肽的核磁共振溶液结构和生物活性。验证了该肽对白色念珠菌、金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的抗菌活性，而未检测到对红细胞或白细胞有明显的裂解作用。
