DNA聚合酶及其缓冲系统对定量实时聚合酶链反应的影响
Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.
作者信息
Wolffs Petra, Grage Halfdan, Hagberg Oskar, Rådström Peter
机构信息
Applied Microbiology, Lund Institute of Technology, Mathematical Statistics, Lund University, SE-221 00 Lund, Sweden.
出版信息
J Clin Microbiol. 2004 Jan;42(1):408-11. doi: 10.1128/JCM.42.1.408-411.2004.
Abstract
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
摘要
一项关于五种DNA聚合酶缓冲液系统对实时PCR影响的研究表明，DNA聚合酶和缓冲液系统的选择都会影响扩增效率以及检测窗口。不同系统数据的分析重复性有明显变化，这使我们得出结论：基于单数据集标准曲线进行定量测量可能会导致显著误差。
相似文献
1
Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.DNA聚合酶及其缓冲系统对定量实时聚合酶链反应的影响
J Clin Microbiol. 2004 Jan;42(1):408-11. doi: 10.1128/JCM.42.1.408-411.2004.
2
Synergy between DNA polymerases increases polymerase chain reaction inhibitor tolerance in forensic DNA analysis.DNA 聚合酶之间的协同作用提高了法医 DNA 分析中聚合酶链反应抑制剂的耐受性。
Anal Biochem. 2010 Oct 15;405(2):192-200. doi: 10.1016/j.ab.2010.06.028. Epub 2010 Jun 19.
3
Influence of DNA polymerases on quantitative PCR results using TaqMan probe format in the LightCycler instrument.DNA聚合酶对在LightCycler仪器中使用TaqMan探针法进行定量PCR结果的影响。
Mol Cell Probes. 2000 Apr;14(2):57-60. doi: 10.1006/mcpr.1999.0284.
4
Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples.九种热稳定DNA聚合酶在存在PCR抑制性样本的情况下介导DNA扩增的能力。
Appl Environ Microbiol. 1998 Oct;64(10):3748-53. doi: 10.1128/AEM.64.10.3748-3753.1998.
5
Amplification efficiency of thermostable DNA polymerases.热稳定DNA聚合酶的扩增效率。
Anal Biochem. 2003 Oct 15;321(2):226-35. doi: 10.1016/s0003-2697(03)00465-2.
6
[Quantitative PCR in the diagnosis of Leishmania].[定量聚合酶链反应在利什曼原虫诊断中的应用]
Parassitologia. 2004 Jun;46(1-2):163-7.
7
A novel buffer system, AnyDirect, can improve polymerase chain reaction from whole blood without DNA isolation.一种新型缓冲系统AnyDirect，无需分离DNA即可改善全血中的聚合酶链反应。
Clin Chim Acta. 2007 May 1;380(1-2):112-7. doi: 10.1016/j.cca.2007.01.019. Epub 2007 Jan 30.
8
Salt-dependent performance variation of DNA polymerases in co-amplification PCR.共扩增PCR中DNA聚合酶的盐依赖性性能变化
Biotechniques. 1996 Jul;21(1):28-30. doi: 10.2144/96211bm04.
9
A distinctive property of Tth DNA polymerase: enzymatic amplification in the presence of phenol.嗜热栖热菌DNA聚合酶的一个独特特性：在苯酚存在下的酶促扩增。
Biotechniques. 1994 Jan;16(1):84-92.
10
Simple sequence repeats amplification.简单序列重复扩增
Methods Mol Biol. 2013;1006:133-8. doi: 10.1007/978-1-62703-389-3_9.
引用本文的文献
1
2
Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.用于研究微生物-宿主相互作用的实时荧光定量PCR技术
Methods Microbiol. 2004;34:255-330. doi: 10.1016/S0580-9517(04)34010-9. Epub 2004 Dec 1.
3
PCR-Based Equine Gene Doping Test for the Australian Horseracing Industry.面向澳大利亚赛马行业的基于聚合酶链式反应的马基因兴奋剂检测
Int J Mol Sci. 2024 Feb 22;25(5):2570. doi: 10.3390/ijms25052570.
4
IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction.IMPLANT：一种利用单终点聚合酶链反应估计植物中转基因拷贝数的新技术。
Plant Methods. 2022 Dec 9;18(1):132. doi: 10.1186/s13007-022-00965-0.
5
Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.污水流行病学在污水处理较差的国家 - 地表水 SARS-CoV-2 RNA 的流行病学指标功能。
Sci Total Environ. 2022 Oct 15;843:156964. doi: 10.1016/j.scitotenv.2022.156964. Epub 2022 Jun 25.
6
Identification of Novel Endogenous Controls for qPCR Normalization in SK-BR-3 Breast Cancer Cell Line.鉴定 SK-BR-3 乳腺癌细胞系 qPCR 归一化的新型内源性对照。
Genes (Basel). 2021 Oct 17;12(10):1631. doi: 10.3390/genes12101631.
7
Comparison of Seven Commercial TaqMan Master Mixes and Two Real-Time PCR Platforms Regarding the Rapid Detection of Porcine DNA.七种商用TaqMan预混液和两种实时荧光定量PCR平台在快速检测猪DNA方面的比较
Food Sci Anim Resour. 2021 Jan;41(1):85-94. doi: 10.5851/kosfa.2020.e80. Epub 2021 Jan 1.
8
PCR inhibition in qPCR, dPCR and MPS-mechanisms and solutions.qPCR、dPCR 和 MPS 中的 PCR 抑制：机制与解决方案。
Anal Bioanal Chem. 2020 Apr;412(9):2009-2023. doi: 10.1007/s00216-020-02490-2. Epub 2020 Feb 12.
9
Essential role of polymerases for assay performance - Impact of polymerase replacement in a well-established assay.聚合酶对检测性能的关键作用——在一项成熟检测中替换聚合酶的影响。
Biomol Detect Quantif. 2018 Nov 14;16:12-20. doi: 10.1016/j.bdq.2018.10.002. eCollection 2018 Dec.
10
Preliminary analysis of New Zealand scampi () diet using metabarcoding.使用代谢条形码技术对新西兰挪威海螯虾（）的饮食进行初步分析。
PeerJ. 2018 Sep 20;6:e5641. doi: 10.7717/peerj.5641. eCollection 2018.
本文引用的文献
1
Evaluation of a rapid, quantitative real-time PCR method for enumeration of pathogenic Candida cells in water.一种用于水中致病性念珠菌细胞计数的快速定量实时聚合酶链反应方法的评估
Appl Environ Microbiol. 2003 Mar;69(3):1775-82. doi: 10.1128/AEM.69.3.1775-1782.2003.
2
Detection and quantification of Escherichia coli O157:H7 in environmental samples by real-time PCR.通过实时聚合酶链反应检测和定量环境样本中的大肠杆菌O157:H7
J Appl Microbiol. 2003;94(3):421-31. doi: 10.1046/j.1365-2672.2003.01848.x.
3
Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria.实时荧光定量PCR结合SYBR Green I或5'-核酸酶分析与基于rDNA的寡核苷酸探针斑点杂交法在定量检测特定粪便细菌中的比较。
Microbiology (Reading). 2003 Jan;149(Pt 1):269-77. doi: 10.1099/mic.0.25975-0.
4
Real-time quantitative PCR.实时定量聚合酶链反应
Methods. 2001 Dec;25(4):383-5. doi: 10.1006/meth.2001.1260.
5
Modeling of 5' nuclease real-time responses for optimization of a high-throughput enrichment PCR procedure for Salmonella enterica.用于优化肠炎沙门氏菌高通量富集PCR程序的5'核酸酶实时反应建模。
J Clin Microbiol. 2002 Jan;40(1):52-60. doi: 10.1128/JCM.40.1.52-60.2002.
6
Quantification of murine IFN-gamma mRNA and protein expression: impact of real-time kinetic RT-PCR using SYBR green I dye.小鼠干扰素-γ mRNA和蛋白表达的定量分析：使用SYBR绿I染料的实时动力学逆转录-聚合酶链反应的影响
Scand J Immunol. 2001 Sep;54(3):285-91. doi: 10.1046/j.1365-3083.2001.00928.x.
7
Application of the 5'-nuclease PCR assay in evaluation and development of methods for quantitative detection of Campylobacter jejuni.5'-核酸酶聚合酶链反应检测法在空肠弯曲菌定量检测方法评估与开发中的应用
Appl Environ Microbiol. 2000 Sep;66(9):4029-36. doi: 10.1128/AEM.66.9.4029-4036.2000.
8
Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification.使用实时聚合酶链反应测定不同猫免疫缺陷病毒分离株的前病毒载量：错配对定量的影响。
Electrophoresis. 1999 Feb;20(2):291-9. doi: 10.1002/(SICI)1522-2683(19990201)20:2<291::AID-ELPS291>3.0.CO;2-R.
9
Detection of pathogenic Yersinia enterocolitica in enrichment media and pork by a multiplex PCR: a study of sample preparation and PCR-inhibitory components.多重PCR检测增菌培养基和猪肉中的致病性小肠结肠炎耶尔森菌：样品制备及PCR抑制成分研究
Int J Food Microbiol. 1998 Dec 8;45(2):93-105. doi: 10.1016/s0168-1605(98)00152-4.
Zotero 插件