Wolffs Petra, Grage Halfdan, Hagberg Oskar, Rådström Peter
Applied Microbiology, Lund Institute of Technology, Mathematical Statistics, Lund University, SE-221 00 Lund, Sweden.
J Clin Microbiol. 2004 Jan;42(1):408-11. doi: 10.1128/JCM.42.1.408-411.2004.
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
一项关于五种DNA聚合酶缓冲液系统对实时PCR影响的研究表明，DNA聚合酶和缓冲液系统的选择都会影响扩增效率以及检测窗口。不同系统数据的分析重复性有明显变化，这使我们得出结论：基于单数据集标准曲线进行定量测量可能会导致显著误差。
