犬类类狼疮性甲营养不良中单核浸润的免疫组织化学评估。

Immunohistochemical evaluation of mononuclear infiltrates in canine lupoid onychodystrophy.

作者信息

Mueller R S, West K, Bettenay S V

机构信息

Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.

出版信息

Vet Pathol. 2004 Jan;41(1):37-43. doi: 10.1354/vp.41-1-37.

DOI:10.1354/vp.41-1-37

PMID:14715966

Abstract

Claw biopsy samples of 11 dogs with lupoid onychodystrophy were evaluated. They were stained with hematoxylin and eosin and with antibodies against CD 3 as a T-cell marker, BLA 36 and HM 57 (CD 79alpha) as B-cell markers, and lysozyme, Mac 387, and major histocompatibility complex (MHC) class II as a marker for histiocytes using an immunoperoxidase and avidin-biotin technique. Inflammatory cells were counted in five high-power fields. The inflammatory infiltrate comprised predominantly B cells and T cells. Macrophages were typically only present in small numbers. CD 3, BLA 36, lysozyme, and MHC class II preserved significant antigenicity during formalin fixation and short decalcification for 24-48 hours, whereas CD 79alpha and particularly Mac 387 seemed to be more susceptible to denaturation by the decalcification process.

摘要

对11只患有类狼疮甲营养不良的犬的爪活检样本进行了评估。样本用苏木精和伊红染色，并使用免疫过氧化物酶和抗生物素蛋白-生物素技术，用抗CD 3（作为T细胞标志物）、抗BLA 36和抗HM 57（CD 79α，作为B细胞标志物）以及抗溶菌酶、抗Mac 387和抗主要组织相容性复合体（MHC）II类（作为组织细胞标志物）的抗体进行染色。在五个高倍视野中对炎性细胞进行计数。炎性浸润主要由B细胞和T细胞组成。巨噬细胞通常仅少量存在。在福尔马林固定和24至48小时的短期脱钙过程中，CD 3、BLA 36、溶菌酶和MHC II类保留了显著的抗原性，而CD 79α，尤其是Mac 387似乎更容易因脱钙过程而变性。