Morita T, Nakamura K, Sawada M, Shimada A, Sato K, Miyata H, Ohama E
Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Minami 4-101, Koyamacho, Tottorishi, Tottori 680-8553, Japan.
Vet Pathol. 2004 Jan;41(1):82-6. doi: 10.1354/vp.41-1-82.
Immunohistochemical study was performed on cerebellar Purkinje cells of two dogs with hypoglycemia using an antibody against the inositol 1,4,5-triphosphate receptor that is identical to the cerebellar Purkinje cell glycoprotein P(400) (P(400)/InsP(3)R). In the cerebellar neocortex of an acute case of hypoglycemia, the P(400)/InsP(3)R staining of hypoglycemic Purkinje cells was heterogeneous: some peripheral dendrites, including spiny branchlets, were negative and others were stained with various intensities, although Purkinje cells were morphologically intact by hematoxylin and eosin (HE) stain. In a chronic case of hypoglycemia, almost all the dendrites of Purkinje cells of both the neo- and archicortex of the cerebellum were not stained with the P(400)/InsP(3)R antibody. This is in contrast to the normal dog where Purkinje cell bodies, axons, and dendrites, including spiny branchlets, are intensely stained by the P(400)/InsP(3)R antibody. These results suggest that P(400)/InsP(3)R immunolabeling of Purkinje cells decreased, despite their morphology being preserved by HE stain, and that the function of P(400)/InsP(3)R, especially in spiny branchlets that receive inputs originating from axon terminals of parallel fibers, may be impaired in hypoglycemia.
使用针对与小脑浦肯野细胞糖蛋白P(400)（P(400)/InsP(3)R）相同的肌醇1,4,5-三磷酸受体的抗体，对两只患有低血糖症的犬的小脑浦肯野细胞进行了免疫组织化学研究。在低血糖症急性病例的小脑新皮质中，低血糖浦肯野细胞的P(400)/InsP(3)R染色是异质性的：一些外周树突，包括棘状小分支，呈阴性，而其他树突则有不同强度的染色，尽管苏木精和伊红（HE）染色显示浦肯野细胞形态完整。在低血糖症慢性病例中，小脑新皮质和旧皮质的浦肯野细胞几乎所有树突均未被P(400)/InsP(3)R抗体染色。这与正常犬相反，在正常犬中，浦肯野细胞体、轴突和树突，包括棘状小分支，均被P(400)/InsP(3)R抗体强烈染色。这些结果表明，尽管HE染色显示浦肯野细胞形态得以保留，但其P(400)/InsP(3)R免疫标记减少，并且在低血糖症中，P(400)/InsP(3)R的功能，尤其是在接受来自平行纤维轴突终末输入的棘状小分支中的功能，可能受损。
