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酵母逆转录病毒样元件Ty1中的RNA分支与去分支

RNA branching and debranching in the yeast retrovirus-like element Ty1.

作者信息

Cheng Zhi, Menees Thomas M

机构信息

School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA.

出版信息

Science. 2004 Jan 9;303(5655):240-3. doi: 10.1126/science.1087023.

DOI:10.1126/science.1087023
PMID:14716018
Abstract

Ty elements of Saccharomyces cerevisiae are long terminal repeat (LTR) retroelements related to retroviruses. Normal levels of Ty1 transposition require Dbr1p, a cellular enzyme that cleaves 2'-5' RNA bonds. We show that Ty1 RNAs lacking identifiable 5' ends accumulate in virus-like particles (VLPs) in dbr1 mutants. Debranching this RNA in vitro with Dbr1p creates an uncapped version of the normal Ty1 RNA 5' end. We show that the 5' nucleotide (nt) of Ty1 RNA forms a 2'-5' bond with a nt near the 3' end of the same RNA, creating a lariat. The properties of the lariat suggest it forms by a novel mechanism and that branching and debranching may play roles in Ty1 reverse transcription at the minus-strand transfer step.

摘要

酿酒酵母的Ty元件是与逆转录病毒相关的长末端重复（LTR）逆转录元件。正常水平的Ty1转座需要Dbr1p，一种切割2'-5'RNA键的细胞酶。我们发现，缺乏可识别5'末端的Ty1 RNA在dbr1突变体的病毒样颗粒（VLP）中积累。用Dbr1p在体外使这种RNA去分支，可产生正常Ty1 RNA 5'末端的无帽版本。我们表明，Ty1 RNA的5'核苷酸（nt）与同一RNA 3'末端附近的一个nt形成2'-5'键，形成一个套索结构。套索结构的特性表明它通过一种新机制形成，并且分支和去分支可能在负链转移步骤的Ty1逆转录中发挥作用。

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