Lee Hye-Youn, Kléber Maurice, Hari Lisette, Brault Véronique, Suter Ueli, Taketo Makoto M, Kemler Rolf, Sommer Lukas
Institute of Cell Biology, Department of Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zurich, Switzerland.
Science. 2004 Feb 13;303(5660):1020-3. doi: 10.1126/science.1091611. Epub 2004 Jan 8.
Wnt signaling has recently emerged as a key factor in controlling stem cell expansion. In contrast, we show here that Wnt/beta-catenin signal activation in emigrating neural crest stem cells (NCSCs) has little effect on the population size and instead regulates fate decisions. Sustained beta-catenin activity in neural crest cells promotes the formation of sensory neural cells in vivo at the expense of virtually all other neural crest derivatives. Moreover, Wnt1 is able to instruct early NCSCs (eNCSCs) to adopt a sensory neuronal fate in a beta-catenin-dependent manner. Thus, the role of Wnt/beta-catenin in stem cells is cell-type dependent.
Wnt信号通路最近已成为控制干细胞增殖的关键因素。相比之下，我们在此表明，迁出的神经嵴干细胞（NCSCs）中Wnt/β-连环蛋白信号激活对细胞群体大小影响很小，而是调节细胞命运决定。神经嵴细胞中持续的β-连环蛋白活性促进体内感觉神经细胞的形成，而几乎以所有其他神经嵴衍生物为代价。此外，Wnt1能够以β-连环蛋白依赖的方式指导早期神经嵴干细胞（eNCSCs）采用感觉神经元命运。因此，Wnt/β-连环蛋白在干细胞中的作用是细胞类型依赖性的。
