Shoshan-Barmatz Varda, Gincel Dan
Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Cell Biochem Biophys. 2003;39(3):279-92. doi: 10.1385/CBB:39:3:279.
Recently, it has been recognized that there is a metabolic coupling between the cytosol and mitochondria, where the outer mitochondrial membrane (OMM), the boundary between these compartments, has important functions. In this crosstalk, mitochondrial Ca2+ homeostasis and ATP production and supply play a major role. The primary transporter of ions and metabolites across the OMM is the voltage-dependent anion channel (VDAC). The interaction of VDAC with Ca2+, ATP glutamate, NADH, and different proteins was demonstrated, and these interactions may regulate OMM permeability. This review includes information on VDAC purification methods, characterization of its channel activity (selectivity, voltage-dependence, conductance), and the regulation of VDAC channel by ligands, such as Ca2+, glutamate and ATP and touches on many aspects of the physiological relevance of VDAC to Ca2+ homeostasis and mitochondria-mediated apoptosis.
最近，人们已经认识到细胞质和线粒体之间存在代谢偶联，其中线粒体外膜（OMM）作为这些区室之间的边界，具有重要功能。在这种相互作用中，线粒体Ca2+稳态以及ATP的产生和供应起着主要作用。离子和代谢物跨线粒体外膜的主要转运体是电压依赖性阴离子通道（VDAC）。已证实VDAC与Ca2+、ATP、谷氨酸、NADH以及不同蛋白质之间存在相互作用，并且这些相互作用可能调节线粒体外膜的通透性。本综述包括有关VDAC纯化方法、其通道活性（选择性、电压依赖性、电导率）的表征，以及配体（如Ca2+、谷氨酸和ATP）对VDAC通道的调节等信息，并涉及VDAC与Ca2+稳态和线粒体介导的细胞凋亡的生理相关性的许多方面。
