Xiao En-Hua, Li Jing-Qing, Huang Jie-Fu
Department of Radiology, the Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China.
World J Gastroenterol. 2004 Jan 15;10(2):190-4. doi: 10.3748/wjg.v10.i2.190.
To evaluate the effects of p53 on apoptosis and proliferation of hepatocellular carcinoma (HCC) cells treated with transcatheter arterial chemoembolization (TACE).
A total of 136 patients with HCC received TACE and other management before surgery were divided into TACE group and non-TACE group. TACE group included 79 patients who had 1-5 courses of TACE before surgery, of them, 11 patients had 1-4 courses of chemotherapy (group A), 33 patients had 1-5 courses of chemotherapy combined with iodized oil (group B), 23 patients had 1-3 courses of chemotherapy, iodized oil and gelatin sponge (group C), 12 patients had 1-3 courses of chemotherapy combined with iodized oil, ethanol and gelatin sponge (group D). Non-TACE group included the remaining 57 patients who had surgery only. The extent of apoptosis was analyzed by transferase mediated dUTP nick end labeling (TUNEL) staining. The expressions of p53, Bcl-2, Bax, Ki-67 and PCNA protein were detected by immunohistochemical method.
P53 protein expressions in trabecular and clear cells in HCC specimens were significantly lower than that in pseudoglandular, solid, poorly differentiated or undifferentiated and sclerosis HCC (P<0.05). Expression of p53 protein in HCC cells increased with the increase of pathological grades (P<0.05), and correlated positively with expressions of Ki-67 and PCNA protein, and negatively with Bcl-2 to Bax protein expression rate and AI (P<0.05). Expression of p53 protein was significantly higher in group A than in groups B, C, D and the non-TACE group, and was higher in group B than in groups C and D, and lower in group D than in the non-TACE group (P<0.05).
Expression of p53 protein can enhance proliferation of HCC cells and suppress apoptosis of HCC cells after TACE.
评估p53对经导管动脉化疗栓塞术（TACE）治疗的肝细胞癌（HCC）细胞凋亡和增殖的影响。
将136例术前接受TACE及其他治疗的HCC患者分为TACE组和非TACE组。TACE组79例，术前接受1 - 5个疗程TACE，其中11例接受1 - 4个疗程化疗（A组），33例接受1 - 5个疗程化疗联合碘化油（B组），23例接受1 - 3个疗程化疗、碘化油和明胶海绵（C组），12例接受1 - 3个疗程化疗联合碘化油、乙醇和明胶海绵（D组）。非TACE组57例，仅接受手术治疗。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）染色分析凋亡程度。采用免疫组织化学方法检测p53、Bcl - 2、Bax、Ki - 67和PCNA蛋白的表达。
HCC标本中梁状及透明细胞的p53蛋白表达明显低于假腺管型、实体型、低分化或未分化及硬化型HCC（P<0.05）。HCC细胞中p53蛋白表达随病理分级增加而升高（P<0.05），与Ki - 67和PCNA蛋白表达呈正相关，与Bcl - 2与Bax蛋白表达率及凋亡指数呈负相关（P<0.05）。A组p53蛋白表达明显高于B、C、D组及非TACE组，B组高于C、D组；D组低于非TACE组（P<0.05）。
p53蛋白表达可促进TACE术后HCC细胞增殖并抑制其凋亡。
