Li Jian-Zhong, Chen Xia, Yang Hua, Wang Shui-Liang, Gong Xue-Lian, Feng Hao, Guo Bao-Yu, Yu Long, Wang Zhu-Gang, Fu Ji-Liang
Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China.
World J Gastroenterol. 2004 Jan 15;10(2):264-7. doi: 10.3748/wjg.v10.i2.264.
Human zinc finger protein 191 (ZNF191) was cloned and characterized as a Kruppel-like transcription factor, which might be relevant to many diseases such as liver cancer, neuropsychiatric and cardiovascular diseases. Although progress has been made recently, the biological function of ZNF191 remains largely unidentified. The aim of this study was to establish a ZNF 191 transgenic mouse model, which would promote the functional study of ZNF191.
Transgene fragments were microinjected into fertilized eggs of mice. The manipulated embryos were transferred into the oviducts of pseudo-pregnant female mice. The offsprings were identified by PCR and Southern blot analysis. ZNF 191 gene expression was analyzed by RT-PCR. Transgenic founder mice were used to establish transgenic mouse lineages. The first generation (F1) and the second generation (F2) mice were identified by PCR analysis. Ten-week transgenic mice were used for pathological examination.
Four mice were identified as carrying copies of ZNF191 gene. The results of RT-PCR showed that ZNF 191 gene was expressed in the liver, testis and brain in one of the transgenic mouse lineages. Genetic analysis of transgenic mice demonstrated that ZNF 191 gene was integrated into the chromosome at a single site and could be transmitted stably. Pathological analysis showed that the expression of ZNF 191 did not cause obvious pathological changes in multiple tissues of transgenic mice.
ZNF 191 transgenic mouse model would facilitate the investigation of biological functions of ZNF191 in vivo.
人类锌指蛋白191（ZNF191）被克隆并鉴定为一种Kruppel样转录因子，它可能与许多疾病相关，如肝癌、神经精神疾病和心血管疾病。尽管最近取得了进展，但ZNF191的生物学功能仍 largely未明。本研究的目的是建立一个ZNF191转基因小鼠模型，这将促进对ZNF191的功能研究。
将转基因片段显微注射到小鼠受精卵中。将操作后的胚胎移植到假孕雌性小鼠的输卵管中。通过PCR和Southern印迹分析鉴定后代。通过RT-PCR分析ZNF191基因表达。利用转基因奠基小鼠建立转基因小鼠品系。通过PCR分析鉴定第一代（F1）和第二代（F2）小鼠。对10周龄的转基因小鼠进行病理检查。
鉴定出4只携带ZNF191基因拷贝的小鼠。RT-PCR结果显示，在其中一个转基因小鼠品系中，ZNF191基因在肝脏、睾丸和大脑中表达。转基因小鼠的遗传分析表明，ZNF191基因整合到染色体的单个位点，并且可以稳定遗传。病理分析表明，ZNF191的表达在转基因小鼠的多个组织中未引起明显的病理变化。
ZNF191转基因小鼠模型将有助于在体内研究ZNF191的生物学功能。
