Gunsé Benet, Garzón Teresa, Barceló Juan
Laboratorio Fisiología Vegetal, Facultad de Ciencias, Universidad Autónoma de Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), Spain.
J Plant Physiol. 2003 Dec;160(12):1447-50. doi: 10.1078/0176-1617-01001.
Aluminum toxicity is a very important factor limiting crop productivity on acid soils. Early effects of aluminum toxicity comprise inhibition of cell division and effects on root elongation. The plasma membrane can be the primary target of aluminum toxicity and thus, vital staining techniques could be a powerful tool in determining effects of metal stress on the plasma membrane. In this paper. we discuss the effects of Al on growth and membrane integrity by staining root tips with a mixture of fluorescein diacetate and propidium iodide. The results show a good correlation between results from growth measurement and the vital staining. From the comparison of the luminosity patterns generated by vital staining it is easy to determine Al-resistant varieties, revealing this technique as a powerful and fast method for determining tolerance to Al in different varieties.
铝毒是限制酸性土壤上作物生产力的一个非常重要的因素。铝毒的早期影响包括抑制细胞分裂和对根伸长的影响。质膜可能是铝毒的主要靶点，因此，活体染色技术可能是确定金属胁迫对质膜影响的有力工具。在本文中，我们通过用荧光素二乙酸酯和碘化丙啶的混合物对根尖进行染色来讨论铝对生长和膜完整性的影响。结果表明，生长测量结果与活体染色结果具有良好的相关性。通过比较活体染色产生的发光模式，可以很容易地确定耐铝品种，表明该技术是一种确定不同品种对铝耐受性的强大而快速的方法。
