Kochavi O, Munichor M, Lichtig C, Meyer E
Department of Ophthalmology, Rambam Medical Center, Haifa, Israel.
Ann Ophthalmol. 1992 Sep;24(9):347-51.
The peroxidase-antiperoxidase staining technique for the antigens S-100, neuron-specific enolase, and alpha 1-antichymotrypsin (alpha 1ACT) was applied to 57 intraocular tumors: 46 malignant melanomas of the uvea, seven retinoblastomas, and four tumors metastasizing to the eye. The staining characteristics of the different intraocular tumors were compared. Staining for S-100 in a fine-needle aspiration biopsy sample taken from a malignant melanoma of the choroid before enucleation of the globe was attempted. The positive staining of a few cells thus obtained suggested that this technique may be helpful in the diagnosis of melanomas. The alpha 1ACT stain used in this study has not been used previously in ophthalmology to our knowledge. We found 60% of malignant melanomas of the choroid stained positively. Another finding was the staining of the retinal pigment epithelium with alpha 1ACT in 30% of eyes with malignant melanoma of the uvea.
采用过氧化物酶-抗过氧化物酶染色技术检测57例眼内肿瘤中的S-100抗原、神经元特异性烯醇化酶和α1-抗糜蛋白酶（α1ACT）：46例葡萄膜恶性黑色素瘤、7例视网膜母细胞瘤和4例转移至眼部的肿瘤。比较了不同眼内肿瘤的染色特征。在眼球摘除术前，尝试对脉络膜恶性黑色素瘤细针穿刺活检样本进行S-100染色。由此获得的少数细胞阳性染色提示该技术可能有助于黑色素瘤的诊断。据我们所知，本研究中使用的α1ACT染色此前未在眼科应用过。我们发现60%的脉络膜恶性黑色素瘤呈阳性染色。另一个发现是，在30%的葡萄膜恶性黑色素瘤患者眼中，视网膜色素上皮细胞被α1ACT染色。
