Tanaka Motoyoshi, Grossman H Barton
Department of Urology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030, USA.
Oncol Rep. 2004 Feb;11(2):537-41.
Connexin 26 (Cx26) encodes a gap junction protein and is a putative tumor suppressor gene. We evaluated the effect of forced expression of Cx26 on three human prostate cancer cell lines, PC-3, LNCap, and DU-145. The three cell lines were infected with a Cx26 adenovirus vector (Ad-Cx26) or a control vector or were mock infected. We tested cell growth, cell cycle, apoptosis, and the efficacy of combined treatment with doxorubicin. Ad-Cx26 infection suppressed the growth of all the cell lines compared with controls and induced cell cycle arrest at the G2/M phase and apoptosis. Ad-Cx26 decreased the expression of Bcl-2. LNCaP cell growth was dramatically suppressed by Ad-Cx26 alone. PC-3 and DU-145 had greater growth suppression with combined gene therapy and chemotherapy than with either Ad-Cx26 or doxorubicin alone. Forced expression of Cx26 suppresses the growth of prostate cancer cells and decreases the expression of Bcl-2. Combining Cx26 gene therapy with doxorubicin results in greater growth suppression.
连接蛋白26（Cx26）编码一种间隙连接蛋白，是一种推定的肿瘤抑制基因。我们评估了Cx26的强制表达对三种人前列腺癌细胞系PC-3、LNCap和DU-145的影响。这三种细胞系分别用Cx26腺病毒载体（Ad-Cx26）、对照载体感染或进行模拟感染。我们检测了细胞生长、细胞周期、细胞凋亡以及与阿霉素联合治疗的效果。与对照相比，Ad-Cx26感染抑制了所有细胞系的生长，并诱导细胞周期停滞于G2/M期和细胞凋亡。Ad-Cx26降低了Bcl-2的表达。单独使用Ad-Cx26可显著抑制LNCaP细胞的生长。与单独使用Ad-Cx26或阿霉素相比，联合基因治疗和化疗对PC-3和DU-145的生长抑制作用更强。Cx26的强制表达可抑制前列腺癌细胞的生长并降低Bcl-2的表达。将Cx26基因治疗与阿霉素联合使用可产生更强的生长抑制作用。
