Ziolkowska Agnieszka, Rucinski Marcin, Neri Giuliano, Di Liddo Rosa, Nussdorfer Gastone G, Malendowicz Ludwik K
Department of Histology and Embryology, Poznan School of Medicine, PL-60781 Poznan, Poland.
Int J Mol Med. 2004 Feb;13(2):215-9.
Beacon gene was recently identified in the rat hypothalamus, and there is evidence that beacon may be involved in the functional regulation of neuroendocrine axes. Reverse transcription-polymerase chain reaction and immunocytochemistry showed the expression of beacon mRNA and protein in the rat adrenal gland, especially in the cortex. Beacon[47-73], at a concentration over 10(-7) M decreased basal aldosterone secretion from dispersed rat zona glomerulosa (ZG) cells, without affecting the ACTH-stimulated one. Basal and agonist-stimulated corticosterone secretion from dispersed zona fasciculata-reticularis cells and catecholamine release from adrenomedullary slices were unaffected by beacon[47-73]. The suppressive effect of beacon[47-73] on aldosterone secretion from ZG cells was abolished by either H-89 or calphostin-C, which are inhibitors of protein kinase A and C signaling cascades. Taken together, these findings allow us to suggest that beacon can be included in the group of regulatory peptides involved in the fine tuning of ZG secretory activity.
信标基因最近在大鼠下丘脑被发现，并且有证据表明信标可能参与神经内分泌轴的功能调节。逆转录-聚合酶链反应和免疫细胞化学显示信标mRNA和蛋白在大鼠肾上腺中表达，尤其是在皮质。浓度超过10(-7) M的信标[47-73]可降低分散的大鼠球状带（ZG）细胞的基础醛固酮分泌，但不影响促肾上腺皮质激素刺激的醛固酮分泌。分散的束状带-网状带细胞的基础和激动剂刺激的皮质酮分泌以及肾上腺髓质切片的儿茶酚胺释放不受信标[47-73]影响。蛋白激酶A和C信号级联的抑制剂H-89或钙磷蛋白C可消除信标[47-73]对ZG细胞醛固酮分泌的抑制作用。综上所述，这些发现使我们认为信标可归入参与ZG分泌活动精细调节的调节肽组。
