Higashide S, Inoue K, Minote H, Tobe T, Mori C, Shiota K
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Nihon Geka Hokan. 1992 Jul 1;61(4):311-9.
The expression and localization of epidermal growth factor (EGF) were investigated immunohistochemically using an anti-EGF receptor antibody in the pancreas of partial pancreatectomized and sham-operated adult rats. In the sham-operated pancreas, immunoreactive products against EGF receptor were only slightly positive in the pancreatic acinar cells. In the partial pancreatectomized pancreas on the fifth day after operation, EGF receptor immunoreactivity was intensely positive in the acinar cells, in some cells lining the intercalated ducts and some basal cells of the acinus, but it was not detected in the pancreas when exogenous EGF was given to the rat after partial pancreatectomy for three days. The present results suggest that EGF receptor is expressed in the regenerating pancreatic tissue, and EGF could be involved in the mechanism of pancreatic regeneration in rats.
使用抗表皮生长因子（EGF）受体抗体，通过免疫组织化学方法研究了部分胰腺切除的成年大鼠和假手术成年大鼠胰腺中EGF的表达和定位。在假手术的胰腺中，针对EGF受体的免疫反应产物在胰腺腺泡细胞中仅呈弱阳性。在术后第5天的部分胰腺切除的胰腺中，EGF受体免疫反应性在腺泡细胞、一些闰管内衬细胞和腺泡的一些基底细胞中呈强阳性，但在部分胰腺切除术后给大鼠注射外源性EGF三天后，胰腺中未检测到。目前的结果表明，EGF受体在再生的胰腺组织中表达，并且EGF可能参与大鼠胰腺再生的机制。
