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二维聚丙烯酰胺凝胶电泳前大鼠个体脑脊液样本制备方法的比较

Comparison of methods for sample preparation of individual rat cerebrospinal fluid samples prior to two-dimensional polyacrylamide gel electrophoresis.

作者信息

Garcia-Rodriguez Sonia, Castilla Sandro Argüelles, Machado Alberto, Ayala Antonio

机构信息

Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González s/n, 41012 Seville, Spain.

出版信息

Biotechnol Lett. 2003 Nov;25(22):1899-903. doi: 10.1023/b:bile.0000003979.92664.11.

DOI:10.1023/b:bile.0000003979.92664.11
PMID:14719824
Abstract

Different pre-treatment methods have been compared for two-dimensional mapping of individual rat cerebrospinal fluid samples based on acetone, trichloroacetic acid/acetone and methanol/acetone precipitation of proteins. Acetone precipitation following incubation with DTT gave the highest protein recovery (72%) and the largest number of protein spots (92 +/- 4) as well as minimizing the time taken.

摘要

基于蛋白质的丙酮沉淀、三氯乙酸/丙酮沉淀和甲醇/丙酮沉淀，对不同的预处理方法进行了比较，用于对大鼠个体脑脊液样本进行二维图谱分析。用二硫苏糖醇（DTT）孵育后进行丙酮沉淀，蛋白质回收率最高（72%），蛋白质斑点数量最多（92±4个），且耗时最短。

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