一种在DNA氧化时释放报告标签的核碱基。

A nucleobase that releases reporter tags upon DNA oxidation.

作者信息

Okamoto Akimitsu, Tanaka Kazuo, Saito Isao

机构信息

Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, and SORST, Japan Science and Technology Corporation, Kyoto 615-8510, Japan.

出版信息

J Am Chem Soc. 2004 Jan 21;126(2):416-7. doi: 10.1021/ja038169a.

DOI:10.1021/ja038169a

PMID:14719909

Abstract

We have developed a novel nucleosbase, edaG, that efficiently releases various reporter units upon one-electron oxidation. The edaG-selective degradation of ODNs was achieved by various mild oxidizing agents. The oxidant-dependent molecular releasing technique is quite useful not only for DNA-based drug releasing systems but also for the detection of long-range hole transport through DNA without time-consuming analysis.

摘要

我们开发了一种新型核碱基edaG，它在单电子氧化后能有效释放各种报告单元。通过各种温和的氧化剂实现了ODNs的edaG选择性降解。这种依赖氧化剂的分子释放技术不仅对基于DNA的药物释放系统非常有用，而且对于无需耗时分析即可检测通过DNA的长程空穴传输也很有用。