Söderström M, Bolling A, Hammarström S
Department of Biochemistry, Stockholm University, Sweden.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):1043-9. doi: 10.1016/0006-291x(92)92309-l.
Leukotriene (LT)C4 synthase is a membrane-bound, specific glutathione transferase which catalyzes the transformation of LTA4 to LTC4. It was originally shown to be present in rodent mastocytoma and basophilic leukemia cells as well as in macrophages. Recently, expression of human LTC4 synthase was demonstrated in platelets (Söderström, M., et al. (1992) Arch. Biochem. Biophys. 294, 70-74). The present report describes the induction of LTC4 synthase activity during differentiation of human erythroleukemia (HEL) cells by the protein kinase C stimulator 12-O-tetradecanoyl phorbol 13-acetate (TPA), ligands of the steroid-thyroid hormone receptor superfamily: all-trans-retinoic acid (RA) and 1 alpha, 25-dihydroxy-vitamin D3 and in addition dimethylsulfoxide (DMSO). TPA was the most powerful inducer of enzyme activity followed by 1 alpha, 25-dihydroxy-vitamin D3 and DMSO. RA did not induce LTC4 synthase activity.
白三烯（LT）C4合成酶是一种膜结合的特异性谷胱甘肽转移酶，可催化LTA4转化为LTC4。最初发现它存在于啮齿动物肥大细胞瘤、嗜碱性白血病细胞以及巨噬细胞中。最近，在血小板中证实了人LTC4合成酶的表达（Söderström，M.等人（1992年）《生物化学与生物物理学报》294，70 - 74）。本报告描述了蛋白激酶C刺激剂12 - O - 十四酰佛波醇13 - 乙酸酯（TPA）、类固醇 - 甲状腺激素受体超家族的配体：全反式维甲酸（RA）和1α，25 - 二羟基维生素D3以及二甲亚砜（DMSO）在人红白血病（HEL）细胞分化过程中对LTC4合成酶活性的诱导作用。TPA是酶活性最强大的诱导剂，其次是1α，25 - 二羟基维生素D3和DMSO。RA不诱导LTC4合成酶活性。
