Mdm2 mRNA expression in salivary gland tumour cell lines.

作者信息

Mantesso Andrea, Loducca Silvia Vanessa Lourenço, Bendit Israel, Garicochea Bernardo, Nunes Fabio Daumas, de Araújo Vera Cavalcanti

机构信息

Department of Oral Pathology, School of Dentistry, University of São Paulo, Av. Lineu Prestes 2227, Cidade Universitária, CEP 05508-900, São Paulo, SP, Brazil.

出版信息

J Oral Pathol Med. 2004 Feb;33(2):96-101. doi: 10.1111/j.1600-0714.2004.00189.x.

DOI:10.1111/j.1600-0714.2004.00189.x

PMID:14720195

Abstract

BACKGROUND

Murine double minute 2 (mdm2) is a cellular protooncogene, which, in conditions of overexpression or amplification, is capable of inactivating the functions of p53, leading to tumorigenesis. Immunoexpression of mdm2 in salivary gland tumours was previously found; however, it was necessary to find out if mdm2 was overexpressed. The aim of this study was to analyse the mRNA expression of mdm2 in salivary gland neoplasms and to correlate it to immunoexpression of p53 and p21 proteins.

METHODS

Specimens of different salivary gland neoplasms were obtained from surgical resections, and cell lineages derived from these tissues were established. RNA extraction was performed and mRNA expression was investigated using reverse transcription-polymerase chain reaction (RT-PCR). Cellular expression of p53 and p21 proteins was investigated by immunofluorescence technique.

RESULTS

Increased expression of mdm2 was found in the majority of cell lines analysed.

CONCLUSIONS

Comparing all results, we postulated that overexpression of mdm2 is related to the tumorigenesis and/or tumour progression of salivary gland neoplasms.

摘要