Tachibana H, Shirahata S, Murakami H
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Biochem Biophys Res Commun. 1992 Dec 15;189(2):625-32. doi: 10.1016/0006-291x(92)92246-t.
A hybridoma line, C5TN, produced human monoclonal antibody of which light chain had N-linked carbohydrate chain within the variable region. Some molecular-weight variants of light chain of the antibody were produced by C5TN variants resistant to cytotoxic effect of concanavalin A. The variant antibodies significantly altered the original cross-reactivity with antigens or lost the ability of antigen binding. The variants variously trimmed their carbohydrate chains by glycosidases, showed the changed reactivity or acquired the ability to bind for antigens. The carbohydrate-deficient antibodies from tunicamycin-treated C5TN and the variant clones behaved in a similar manner on antigen-binding reactivity. Furthermore, comparison of antibodies of which light chains have carbohydrate chains sensitive and resistant to some glycosidases showed that carbohydrate chain in variable region of light chain can influence their reactivity with antigen.
一种杂交瘤细胞系C5TN产生了人单克隆抗体，其轻链在可变区内含有N-连接糖链。对伴刀豆球蛋白A的细胞毒性作用具有抗性的C5TN变体产生了该抗体轻链的一些分子量变体。变体抗体显著改变了与抗原的原始交叉反应性，或丧失了抗原结合能力。这些变体通过糖苷酶不同程度地修剪了它们的糖链，表现出改变的反应性或获得了与抗原结合的能力。来自衣霉素处理的C5TN和变体克隆的无糖基化抗体在抗原结合反应性上表现出相似的行为。此外，对轻链糖链对某些糖苷酶敏感和抗性的抗体进行比较表明，轻链可变区内的糖链可以影响它们与抗原的反应性。
