[细胞周期蛋白D1在脑胶质瘤中的表达及其意义]

[Expression of cyclin D1 in brain gliomas and its significance].

作者信息

Tan Ping-Guo, Xing Zhou, Li Ze-Qun

机构信息

Department of Neurosurgery, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, 510120, P.R.China.

出版信息

Ai Zheng. 2004 Jan;23(1):63-5.

PMID:14720377

Abstract

BACKGROUND & OBJECTIVE: Overexpression of cyclin D1 was shown in many tumors. The excessive expression of cyclin D1 is an important cause of many tumors. But there are still some controversies of whether the overexpression of cyclin D1 exists in brain gliomas. This study was to determine the expression level of cyclin D1 in glioma tissues of human brain, and to analyze the relationship of cyclin D1 with the malignancy and prognosis of gliomas.

METHODS

The expression levels of cyclin D1 in 84 specimens were determined by SP immunohistochemical assay. The correlation of expression intensity of cyclin D1, positive cell ratio of glioma tissues with the tumors malignancy, and the prognosis of the patients was analyzed.

RESULTS

(1) The average percentages of cyclin D1 positive cells were (9.82+/-9.75)% and (27.45+/-21.03)% in the low grade gliomas and the high grade gliomas, respectively. There was significant difference between two groups (P< 0.01). (2) The cyclin D1 positive ratios were 31.25% (10/32) and 61.53% (32/52) in the low grade gliomas and the high grade gliomas, respectively. There was significant difference between two groups (P< 0.01). (3) The cyclin D1 positive ratios were 76.19% (16/21) and 24.00% (6/25) in recurrence group and non-recurrence group,respectively. There was significant difference between two groups (P< 0.01). (4) The cyclin D1 positive ratios were 66.67% (14/21) and 32.00%(8/25) in dead group and survival group, respectively. There was significant difference between two groups (P< 0.05). In dead group, the cyclin D1 positive ratios were 86.66%(13/15) and 16.66%(1/6) in the high grade gliomas and the low grade gliomas, respectively. There was significant difference between two groups (P< 0.05).

CONCLUSIONS

(1) The expression of cyclin D1 increased with the increased grade of glioma. (2) The higher cyclin D1 expressed, the worse prognosis the patients had. (3) The expression of cyclin D1 can act as a biological marker in evaluating malignancy of gliomas and prognosis of patients.

摘要

背景与目的

细胞周期蛋白D1在多种肿瘤中呈过表达。细胞周期蛋白D1的过度表达是多种肿瘤的重要成因。但细胞周期蛋白D1在脑胶质瘤中是否存在过表达仍存在一些争议。本研究旨在测定人脑胶质瘤组织中细胞周期蛋白D1的表达水平，并分析细胞周期蛋白D1与胶质瘤恶性程度及预后的关系。

方法

采用SP免疫组化法检测84例标本中细胞周期蛋白D1的表达水平。分析细胞周期蛋白D1表达强度、胶质瘤组织阳性细胞率与肿瘤恶性程度及患者预后的相关性。

结果

（1）低级别胶质瘤和高级别胶质瘤中细胞周期蛋白D1阳性细胞的平均百分比分别为（9.82±9.75）%和（27.45±21.03）%。两组间差异有统计学意义（P<0.01）。（2）低级别胶质瘤和高级别胶质瘤中细胞周期蛋白D1阳性率分别为31.25%（10/32）和61.53%（32/52）。两组间差异有统计学意义（P<0.01）。（3）复发组和未复发组中细胞周期蛋白D1阳性率分别为76.19%（16/21）和24.00%（6/25）。两组间差异有统计学意义（P<0.01）。（4）死亡组和生存组中细胞周期蛋白D1阳性率分别为66.67%（14/21）和32.00%（8/25）。两组间差异有统计学意义（P<0.05）。在死亡组中，高级别胶质瘤和低级别胶质瘤中细胞周期蛋白D1阳性率分别为86.66%（13/15）和16.66%（1/6）。两组间差异有统计学意义（P<0.05）。