[上皮性卵巢癌中p21（WAF1）的表达及其与p53和PCNA蛋白的关系]

[Expression of p21(WAF1) and its relationship with p53 and PCNA protein in epithelial ovarian cancer].

作者信息

Yan Xiao-Jian, Liang Li-Zhi, Li Dao-Cheng, Li Jing-Lue, Zhang Chang-Qing, Yuan Song-Hua

机构信息

Department of Gynecology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, P.R.China.

出版信息

Ai Zheng. 2004 Jan;23(1):74-80.

PMID:14720380

Abstract

BACKGROUND & OBJECTIVE: Recent researches manifested that down-regulation of p21(WAF1) had relationship with carcinogenesis and development in various tumors, but its association with epithelial ovarian cancer (EOC) was not clear. This study was designed to investigate the role of p21(WAF1) in the tumorigenesis and development of EOC and its relationship with p53 and proliferating cell nuclear antigen (PCNA) protein.

METHODS

Fifty-five EOC tissues, 32 benign ovarian tumor tissues, and 30 normal ovarian tissues were collected. Reverse transcription polymerase chain reaction (RT-PCR) was applied to determine the p21(WAF1)mRNA expression. Immunohistochemistry was applied to examine the protein expression of p21(WAF1), p53, and PCNA. The relationship between the expression of these markers and the clinicopathological characteristics, prognosis of the patients was analyzed.

RESULTS

The positive rates of p21(WAF1)mRNA in EOC, benign ovarian tumor, and normal ovary were 40%, 56.25%, and 73.33%, respectively (P=0.012). The positive rates of p21(WAF1) protein were 36.36%, 56.25%, and 80%, respectively (P=0.001). The positive expression rates of p21(WAF1)mRNA and its protein in EOC were lower than those of the other two groups, while the positive expression rates of p53 and PCNA protein in EOC were higher than those of the other two groups (P< 0.05). Expression of p21(WAF1)mRNA had positive relation to its protein, negative relation to PCNA protein, no relation to p53 protein, while expression of p21(WAF1) protein had negative relation to p53 and PCNA protein in EOC. Low-expression of p21(WAF1) protein was associated with advanced FIGO stage (P=0.032), but not with age, histological type, pathological grade, and remnant tumor (P >0.05). There was no relationship between p21(WAF1)mRNA and former parameters (P >0.05). Univariate analysis showed that the patients with low-expression of p21(WAF1)mRNA and p21(WAF1) protein had poor prognosis (P< 0.05).

CONCLUSION

p21(WAF1) is down-regulated in EOC. p21(WAF1) might be able to be used as a marker to predict the prognosis of patients with EOC.

摘要

背景与目的

近期研究表明，p21（WAF1）表达下调与多种肿瘤的发生发展相关，但其与上皮性卵巢癌（EOC）的关系尚不清楚。本研究旨在探讨p21（WAF1）在上皮性卵巢癌发生发展中的作用及其与p53和增殖细胞核抗原（PCNA）蛋白的关系。

方法

收集55例上皮性卵巢癌组织、32例卵巢良性肿瘤组织及30例正常卵巢组织。采用逆转录聚合酶链反应（RT-PCR）检测p21（WAF1）mRNA表达，免疫组织化学法检测p21（WAF1）、p53和PCNA蛋白表达。分析这些标志物表达与患者临床病理特征、预后的关系。

结果

上皮性卵巢癌、卵巢良性肿瘤及正常卵巢组织中p21（WAF1）mRNA阳性率分别为40%、56.25%和73.33%（P=0.012）；p21（WAF1）蛋白阳性率分别为36.36%、56.25%和80%（P=0.001）。上皮性卵巢癌中p21（WAF1）mRNA及其蛋白阳性表达率低于其他两组，而p53和PCNA蛋白阳性表达率高于其他两组（P<0.05）。上皮性卵巢癌中p21（WAF1）mRNA表达与其蛋白呈正相关，与PCNA蛋白呈负相关，与p53蛋白无关；p21（WAF1）蛋白表达与p53和PCNA蛋白呈负相关。p21（WAF1）蛋白低表达与国际妇产科联盟（FIGO）分期晚相关（P=0.032），与年龄、组织学类型、病理分级及残留肿瘤无关（P>0.05）。p21（WAF1）mRNA与上述参数无关（P>0.05）。单因素分析显示，p21（WAF1）mRNA和p21（WAF1）蛋白低表达患者预后较差（P<0.05）。