Wong J W C, Xiang L, Gu X Y, Zhou L X
Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, PR China.
Chemosphere. 2004 Apr;55(1):101-7. doi: 10.1016/j.chemosphere.2003.11.022.
The effect of using FeS2 as an energy source, on the bioleaching of heavy metals (Zn, Cr, Cu, Pb and Ni) and nutrients (nitrogen and phosphorus) from anaerobically digested sludge using isolated indigenous iron-oxidizing bacteria was investigated in this paper. Addition of FeS2 in the range of 0.5-4.0 g l(-1) accelerated the acidification of sludge and raised the oxidation-reduction potential of sludge medium with an inoculation of 15% (v/v) of active bacteria, thus resulting in an overall increase in metal dissolution efficiency. After 16 days of bioleaching at 28 degrees C and an initial pH of 3.0, up to 99% of Zn, 65% of Cr, 74% of Cu, 58% of Pb and 84% of Ni can be removed from the sludge. In contrast, only 94% of Zn, 12% of Cr, 21% of Cu, 32% of Pb and 38% of Ni were leached out in the control without inoculation of iron-oxidizing bacteria and the addition of FeS2. Less than 15% of nitrogen and 6% of phosphorous were lost after 16 days of bioleaching when using FeS2 as the energy source. Comparing to 39% and 45% loss respectively for these two nutrients when using FeSO4.7H2O as the energy source, FeS2 appears to be a more suitable energy source for preserving nutrients in sludge while removing heavy metals from sludge.
本文研究了使用二硫化铁（FeS2）作为能源，对利用分离出的本地铁氧化细菌从厌氧消化污泥中生物浸出重金属（锌、铬、铜、铅和镍）及养分（氮和磷）的影响。添加0.5 - 4.0 g l(-1)范围内的FeS2，并接种15%（v/v）的活性细菌，加速了污泥的酸化，提高了污泥培养基的氧化还原电位，从而使金属溶解效率总体提高。在28℃、初始pH值为3.0的条件下进行16天生物浸出后，污泥中高达99%的锌、65%的铬、74%的铜、58%的铅和84%的镍可被去除。相比之下，在未接种铁氧化细菌且未添加FeS2的对照实验中，仅94%的锌、12%的铬、21%的铜、32%的铅和38%的镍被浸出。以FeS2作为能源进行16天生物浸出后，氮的损失不到15%，磷的损失为6%。与以七水硫酸亚铁（FeSO4·7H2O）作为能源时这两种养分分别损失39%和45%相比，FeS2似乎是一种在从污泥中去除重金属的同时更适合保留污泥中养分的能源。
