Hoeger Ulrich, Abe Hiroki
Laboratory of Marine Biochemistry, Department of Aquatic Bioscience, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan.
Comp Biochem Physiol A Mol Integr Physiol. 2004 Jan;137(1):161-71. doi: 10.1016/s1095-6433(03)00286-1.
The free amino acid pool was measured in the body wall muscle and in coelomic cells (eleocytes) of the polychaete Nereis japonica following adaptation to salinities between 6 and 44 per thousand. Beta-Alanine and glycine were the major amino acids comprising 35-60% of the total free amino acid pool in the body wall. In eleocytes, glutamate and lysine in addition to beta-alanine and glycine were the dominant free amino acids. In the body wall, the concentrations of beta-alanine were closely correlated with the ambient salinity between 12 and 35 per thousand. The concentrations of glycine rose initially but remained unchanged at concentrations above 26 per thousand. In both body wall and eleocytes, the mean total primary amine concentrations were correlated with the ambient salinities between 12 and 35 per thousand. The sum of amino acids determined by HPLC showed the same correlation in both tissues, but accounted only for 60-85% of the concentrations of total primary amines. The total protein content of the body wall was slightly higher at 44 per thousand compared to the lower salinities indicating dehydration of the tissues. Eleocytes swell at 6 per thousand and showed irregular amino acid concentrations indicating a loss of metabolic integrity.
在多毛纲动物日本沙蚕适应千分之六至千分之四十四的盐度后，对其体壁肌肉和体腔细胞（血细胞）中的游离氨基酸库进行了测量。β-丙氨酸和甘氨酸是主要氨基酸，占体壁中游离氨基酸库总量的35%-60%。在血细胞中，除了β-丙氨酸和甘氨酸外，谷氨酸和赖氨酸是主要的游离氨基酸。在体壁中，β-丙氨酸的浓度与千分之十二至千分之三十五的环境盐度密切相关。甘氨酸的浓度最初上升，但在高于千分之二十六的浓度时保持不变。在体壁和血细胞中，平均总伯胺浓度与千分之十二至千分之三十五的环境盐度相关。通过高效液相色谱法测定的氨基酸总和在两种组织中显示出相同的相关性，但仅占总伯胺浓度的60%-85%。与较低盐度相比，体壁的总蛋白质含量在千分之四十四时略高，表明组织脱水。血细胞在千分之六时膨胀，氨基酸浓度不规则，表明代谢完整性丧失。
