豇豆花叶病毒的运动蛋白在体外能结合鸟苷三磷酸（GTP）和单链核酸。
The movement protein of cowpea mosaic virus binds GTP and single-stranded nucleic acid in vitro.
作者信息
Carvalho C M, Pouwels J, van Lent J W M, Bisseling T, Goldbach R W, Wellink J
机构信息
Laboratory of Virology, Department of Plant Sciences, Wageningen University, 6709 PD Wageningen, The Netherlands.
出版信息
J Virol. 2004 Feb;78(3):1591-4. doi: 10.1128/jvi.78.3.1591-1594.2004.
Abstract
The movement protein (MP) of Cowpea mosaic virus forms tubules in plasmodesmata to enable the transport of mature virions. Here it is shown that the MP is capable of specifically binding riboguanosine triphosphate and that mutational analysis suggests that GTP binding plays a role in the targeted transport of the MP. Furthermore, the MP is capable of binding both single-stranded RNA and single-stranded DNA in a non-sequence-specific manner, and the GTP- and RNA-binding sites do not overlap.
摘要
豇豆花叶病毒的运动蛋白（MP）在胞间连丝中形成小管，以促进成熟病毒粒子的运输。本文表明，MP能够特异性结合三磷酸核糖鸟苷，并且突变分析表明，GTP结合在MP的靶向运输中发挥作用。此外，MP能够以非序列特异性方式结合单链RNA和单链DNA，且GTP结合位点与RNA结合位点不重叠。
相似文献
1
The movement protein of cowpea mosaic virus binds GTP and single-stranded nucleic acid in vitro.豇豆花叶病毒的运动蛋白在体外能结合鸟苷三磷酸（GTP）和单链核酸。
J Virol. 2004 Feb;78(3):1591-4. doi: 10.1128/jvi.78.3.1591-1594.2004.
2
The C-terminal region of the movement protein of Cowpea mosaic virus is involved in binding to the large but not to the small coat protein.豇豆花叶病毒运动蛋白的C末端区域参与与大的衣壳蛋白结合，但不与小的衣壳蛋白结合。
J Gen Virol. 2003 Aug;84(Pt 8):2271-2277. doi: 10.1099/vir.0.19101-0.
3
The NTP-binding motif in cowpea mosaic virus B polyprotein is essential for viral replication.豇豆花叶病毒B多聚蛋白中的NTP结合基序对病毒复制至关重要。
J Gen Virol. 1994 Nov;75 ( Pt 11):3167-76. doi: 10.1099/0022-1317-75-11-3167.
4
The movement proteins of cowpea mosaic virus and cauliflower mosaic virus induce tubular structures in plant and insect cells.豇豆花叶病毒和花椰菜花叶病毒的运动蛋白可在植物和昆虫细胞中诱导管状结构的形成。
J Gen Virol. 1996 Nov;77 ( Pt 11):2857-64. doi: 10.1099/0022-1317-77-11-2857.
5
Proteomic analysis of the plasma membrane-movement tubule complex of cowpea mosaic virus.豇豆花叶病毒质膜运动小管复合体的蛋白质组学分析。
Arch Virol. 2016 May;161(5):1309-14. doi: 10.1007/s00705-016-2757-3. Epub 2016 Jan 18.
6
Studies on the movement of cowpea mosaic virus using the jellyfish green fluorescent protein.利用水母绿色荧光蛋白对豇豆花叶病毒运动的研究。
Virology. 1998 Mar 1;242(1):22-7. doi: 10.1006/viro.1997.8982.
7
Cell-to-cell movement of Alfalfa mosaic virus can be mediated by the movement proteins of Ilar-, bromo-, cucumo-, tobamo- and comoviruses and does not require virion formation.苜蓿花叶病毒的细胞间移动可由等轴不稳环斑病毒属、雀麦花叶病毒属、黄瓜花叶病毒属、烟草花叶病毒属和豇豆花叶病毒属的移动蛋白介导，且不需要病毒粒子的形成。
Virology. 2006 Mar 1;346(1):66-73. doi: 10.1016/j.virol.2005.10.024. Epub 2005 Nov 28.
8
Identification of distinct steps during tubule formation by the movement protein of Cowpea mosaic virus.
J Gen Virol. 2003 Dec;84(Pt 12):3485-3494. doi: 10.1099/vir.0.19553-0.
9
The cytoskeleton and the secretory pathway are not involved in targeting the cowpea mosaic virus movement protein to the cell periphery.细胞骨架和分泌途径不参与将豇豆花叶病毒运动蛋白靶向细胞周边。
Virology. 2002 May 25;297(1):48-56. doi: 10.1006/viro.2002.1424.
10
Studies on the origin and structure of tubules made by the movement protein of Cowpea mosaic virus.
J Gen Virol. 2004 Dec;85(Pt 12):3787-3796. doi: 10.1099/vir.0.80497-0.
引用本文的文献
1
Groundnut bud necrosis virus encoded movement protein NSm binds to GTP and ATP.花生芽坏死病毒编码的运动蛋白NSm与鸟苷三磷酸（GTP）和三磷酸腺苷（ATP）结合。
3 Biotech. 2025 May;15(5):146. doi: 10.1007/s13205-025-04305-6. Epub 2025 Apr 30.
2
The Arabidopsis synaptotagmin SYTA regulates the cell-to-cell movement of diverse plant viruses.拟南芥突触结合蛋白SYTA调控多种植物病毒的细胞间移动。
Front Plant Sci. 2014 Nov 6;5:584. doi: 10.3389/fpls.2014.00584. eCollection 2014.
3
Host and viral RNA-binding proteins involved in membrane targeting, replication and intercellular movement of plant RNA virus genomes.参与植物RNA病毒基因组膜靶向、复制和细胞间运动的宿主和病毒RNA结合蛋白。
Front Plant Sci. 2014 Jul 7;5:321. doi: 10.3389/fpls.2014.00321. eCollection 2014.
4
Movement protein Pns6 of rice dwarf phytoreovirus has both ATPase and RNA binding activities.水稻矮缩病毒运动蛋白 Pns6 具有 ATP 酶和 RNA 结合活性。
PLoS One. 2011;6(9):e24986. doi: 10.1371/journal.pone.0024986. Epub 2011 Sep 20.
5
Replication-independent long-distance trafficking by viral RNAs in Nicotiana benthamiana.烟草中病毒RNA的非复制依赖性长距离运输
Plant Cell. 2007 Apr;19(4):1179-91. doi: 10.1105/tpc.107.050088. Epub 2007 Apr 6.
本文引用的文献
1
TRANSPORT OF PROTEINS AND NUCLEIC ACIDS THROUGH PLASMODESMATA.蛋白质和核酸通过胞间连丝的运输
Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:27-50. doi: 10.1146/annurev.arplant.48.1.27.
2
Identification of distinct steps during tubule formation by the movement protein of Cowpea mosaic virus.
J Gen Virol. 2003 Dec;84(Pt 12):3485-3494. doi: 10.1099/vir.0.19553-0.
3
The C-terminal region of the movement protein of Cowpea mosaic virus is involved in binding to the large but not to the small coat protein.豇豆花叶病毒运动蛋白的C末端区域参与与大的衣壳蛋白结合，但不与小的衣壳蛋白结合。
J Gen Virol. 2003 Aug;84(Pt 8):2271-2277. doi: 10.1099/vir.0.19101-0.
4
Dynamics and mechanics of the microtubule plus end.微管正端的动力学与力学
Nature. 2003 Apr 17;422(6933):753-8. doi: 10.1038/nature01600.
5
Plasmodesmata: dynamic regulation and role in macromolecular cell-to-cell signaling.胞间连丝：动态调控及其在细胞间大分子信号传导中的作用
Curr Opin Plant Biol. 2002 Dec;5(6):543-52. doi: 10.1016/s1369-5266(02)00295-9.
6
Cell-to-Cell and Long-Distance Transport of Viruses in Plants.植物中病毒的细胞间和长距离运输
Plant Cell. 1996 Oct;8(10):1669-1681. doi: 10.1105/tpc.8.10.1669.
7
In vivo accumulation of Broad bean wilt virus 2 VP37 protein and its ability to bind single-stranded nucleic acid.蚕豆萎蔫病毒2 VP37蛋白的体内积累及其与单链核酸结合的能力。
Arch Virol. 2002 May;147(5):917-28. doi: 10.1007/s00705-001-0782-2.
8
Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein.基因I是花椰菜花叶病毒一个潜在的细胞间运动位点，编码一种RNA结合蛋白。
Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2476-80. doi: 10.1073/pnas.88.6.2476.
9
Mutational analysis of the cowpea mosaic virus movement protein.豇豆花叶病毒运动蛋白的突变分析
Virology. 2000 Feb 15;267(2):199-208. doi: 10.1006/viro.1999.0087.
10
Engineering cowpea mosaic virus RNA-2 into a vector to express heterologous proteins in plants.将豇豆花叶病毒RNA - 2改造为载体以在植物中表达异源蛋白。
Virology. 2000 Feb 15;267(2):159-73. doi: 10.1006/viro.1999.0126.
Zotero 插件