Yacoubi Loueslati B, Ennafaa H, Ben Amor M, Heyer E, Langaney A, Ben Ayed F, Ben Ammar Elgaaied A
Laboratoire d'Anthropologie Biologique, CNRS, Musée de l'Homme Paris.
Arch Inst Pasteur Tunis. 1998 Jan-Apr;75(1-2):9-18.
Jerba is an island situated in the South-East of Tunisia were some ethnic groups (Arabs, Berbers, Blacks, Jewishs and others) cohabit for centuries. The religion and cultural differences have represented an obstacle to a mixture between these groups. In order to evaluate the genetic differentiation between the muslim groups (Arabs, Berbers and Blacks), we have analysed the polymorphism of a mitochondrial DNA coding region. The cytochrome oxydase coding region (COII) was amplified by PCR in 57 Arabs, 42 Berbers and 16 Blacks. The amplified products were analysed by Restriction Fragment Length Polymorphism (RFLP). Genetic distances were calculated by using the AMOVA program. The values of these distances were significantly different between Arabs and Blacks, and between Berbers and Blacks but not between Arabs and Berbers. So That, to refine the evaluation of genetic diversity between Arabs and Berbers, we have analysed the polymorphism of a second mitochondrial coding region which encodes for the fifth unit of NADH deshydrogenase (ND5). Eleven haplotypes were defined from the resulting data of mitochondrial COII and ND5 polymorphism and a significant genetic distance between Arabs and Berbers was computed.
杰尔巴岛是位于突尼斯东南部的一个岛屿，几个世纪以来，一些族群（阿拉伯人、柏柏尔人、黑人、犹太人等）共同生活在那里。宗教和文化差异一直是这些群体之间融合的障碍。为了评估穆斯林群体（阿拉伯人、柏柏尔人和黑人）之间的遗传分化，我们分析了线粒体DNA编码区的多态性。通过聚合酶链反应（PCR）在57名阿拉伯人、42名柏柏尔人和16名黑人中扩增了细胞色素氧化酶编码区（COII）。扩增产物通过限制性片段长度多态性（RFLP）进行分析。使用分子变异分析（AMOVA）程序计算遗传距离。阿拉伯人与黑人之间以及柏柏尔人与黑人之间的这些距离值存在显著差异，但阿拉伯人与柏柏尔人之间没有显著差异。因此，为了更精确地评估阿拉伯人和柏柏尔人之间的遗传多样性，我们分析了编码烟酰胺腺嘌呤二核苷酸脱氢酶第五亚基（ND5）的第二个线粒体编码区的多态性。从线粒体COII和ND5多态性的结果数据中定义了11种单倍型，并计算出阿拉伯人和柏柏尔人之间存在显著的遗传距离。
