Keith L D, Crabbe J C
Research Service, Veterans Administration Medical Center, Portland, OR 97201.
Alcohol. 1992 Nov-Dec;9(6):529-33. doi: 10.1016/0741-8329(92)90092-o.
The intent of this study was to determine whether chronic ethanol (EtOH) vapor inhalation, with or without adjunct pyrazole (PYR) administration, was stressful in mice, as defined by increases in plasma corticosterone (CORT) concentration. Mice were randomly assigned to groups differentiated both on the basis of EtOH vapor exposure and the presence or absence of PYR administration. Blood samples for blood EtOH concentration (BEC) and plasma CORT concentration were obtained from mice after 72-96 hours of treatment. Mice were sacrificed after 96 hours of treatment and body and adrenal weight determined. BEC was significantly higher in PYR-treated animals and animals treated with the higher EtOH vapor concentration. Plasma CORT was elevated in proportion to BEC; however, other nonspecific stresses, in particular that of PYR administration, also elevated plasma CORT. Nonspecific stresses associated with this protocol may reduce the generality of these observations. Nevertheless, the high correlation between BEC and plasma CORT concentration in the PYR groups indicates that, with suitable control groups, the PYR-EtOH vapor inhalation approach is viable for studies concerned with EtOH effects on hypothalamic-anterior pituitary-adrenocortical function.
本研究的目的是确定慢性吸入乙醇（EtOH）蒸汽（无论是否辅以吡唑（PYR）给药）对小鼠是否具有应激作用，应激作用的定义为血浆皮质酮（CORT）浓度升高。小鼠被随机分为不同组，这些组在乙醇蒸汽暴露情况以及是否给予吡唑方面存在差异。在治疗72 - 96小时后从小鼠采集血样，检测血液乙醇浓度（BEC）和血浆CORT浓度。治疗96小时后处死小鼠，测定体重和肾上腺重量。接受PYR治疗的动物以及暴露于较高乙醇蒸汽浓度下的动物，其BEC显著更高。血浆CORT与BEC成比例升高；然而，其他非特异性应激，尤其是给予PYR这一应激，也会使血浆CORT升高。与该实验方案相关的非特异性应激可能会降低这些观察结果的普遍性。尽管如此，PYR组中BEC与血浆CORT浓度之间的高度相关性表明，设置合适的对照组后，PYR - 乙醇蒸汽吸入法对于研究乙醇对下丘脑 - 垂体前叶 - 肾上腺皮质功能的影响是可行的。
