Muehleman Carol, Majumdar Sharmila, Issever Ahi Sema, Arfelli Fulvia, Menk Ralf Hendrik, Rigon Luigi, Heitner Gabriele, Reime Bernd, Metge Joachim, Wagner Andreas, Kuettner Klaus E, Mollenhauer Juergen
Department of Anatomy and Cell Biology, Rush Medical College, IL 60612, Chicago, USA.
Osteoarthritis Cartilage. 2004 Feb;12(2):97-105. doi: 10.1016/j.joca.2003.10.001.
To determine the feasibility of detecting the structural orientation in cartilage with Diffraction Enhanced X-Ray Imaging.
Human tali and femoral head specimens were Diffraction Enhanced X-Ray Imaged (DEI) at the SYRMEP beamline at Elettra at various energy levels to detect the architectural arrangement of collagen within cartilage. DEI utilizes a monochromatic and highly collimated beam, with an analyzer crystal that selectively weights out photons according to the angle they have been deviated with respect to the original direction. This provides images of very high contrast, and with the rejection of X-ray scatter.
DEI allowed the visualization of articular cartilage and a structural orientation, resembling arcades, within.
Our diffraction enhanced images represent the first radiographic detection of the structural orientation in cartilage. Our data are in line with previous studies on the structural organization of joint cartilage. They confirm the model of a vaulting system of collagen fiber bundles interrupted by proteoglycan aggregates.
确定采用衍射增强X射线成像检测软骨结构取向的可行性。
在Elettra的SYRMEP光束线对人类距骨和股骨头标本进行不同能量水平的衍射增强X射线成像（DEI），以检测软骨内胶原蛋白的结构排列。DEI使用单色且高度准直的光束，以及一个分析晶体，该晶体根据光子相对于原始方向的偏离角度选择性地筛选光子。这提供了对比度非常高且去除了X射线散射的图像。
DEI能够显示关节软骨及其内部类似拱廊的结构取向。
我们的衍射增强图像代表了软骨结构取向的首次放射学检测。我们的数据与先前关于关节软骨结构组织的研究一致。它们证实了由蛋白聚糖聚集体中断的胶原纤维束拱形系统模型。
