Jessen T E, Ødum L
Department of Clinical Biochemistry, Holbaek, Sygehus Vestsjaelland, DK- 4300, Holaek, Denmark.
Osteoarthritis Cartilage. 2004 Feb;12(2):142-8. doi: 10.1016/j.joca.2003.10.004.
To investigate the role of tumor necrosis factor stimulated gene 6 (TSG-6) and metal ions in the coupling of inter-alpha-trypsin inhibitor (ITI) to hyaluronan in human synovial fluid.
The concentration of ITI heavy chains bound to hyaluronan was determined by a two-step electrophoretic technique. Synovial fluid, TSG-6 depleted synovial fluid and metal chelated synovial fluid were tested for their ability to support the coupling of ITI heavy chains to hyaluronan.
When synovial fluid was mixed with an ITI-source (serum or purified ITI), coupling of ITI heavy chains to hyaluronan took place. TSG-6 immunodepleated synovial fluid lost the coupling activity, but addition of recombinant TSG-6 restored the activity. EDTA inhibited the coupling activity, but combinations of the metal-ion chelators Mg-EGTA and Ca-EGTA demonstrated, that Ca++ is essential for the coupling of ITI heavy chains to hyaluronan.
Tumor necrosis factor stimulated gene 6 (TSG-6) and calcium ions are both essential for the coupling of inter-alpha-trypsin inhibitor to hyaluronan in human synovial fluid.
研究肿瘤坏死因子刺激基因6（TSG-6）和金属离子在人滑液中α-胰蛋白酶抑制剂（ITI）与透明质酸偶联中的作用。
采用两步电泳技术测定与透明质酸结合的ITI重链浓度。检测滑液、TSG-6缺失的滑液和金属螯合的滑液支持ITI重链与透明质酸偶联的能力。
当滑液与ITI来源（血清或纯化的ITI）混合时，ITI重链与透明质酸发生偶联。TSG-6免疫缺失的滑液失去偶联活性，但添加重组TSG-6可恢复活性。EDTA抑制偶联活性，但金属离子螯合剂Mg-EGTA和Ca-EGTA的组合表明，Ca++对于ITI重链与透明质酸的偶联至关重要。
肿瘤坏死因子刺激基因6（TSG-6）和钙离子对于人滑液中α-胰蛋白酶抑制剂与透明质酸的偶联均至关重要。
