氯对雌激素类化学物质减少的影响。

Effects of chlorine on the decrease of estrogenic chemicals.

作者信息

Lee Byoung-Cheun, Kamata Motoyuki, Akatsuka Yasushi, Takeda Makoto, Ohno Koichi, Kamei Tasuku, Magara Yasumoto

机构信息

Graduate School of Engineering, Hokkaido University, N13, W8, Kita-ku, Sapporo 060-8628, Japan.

出版信息

Water Res. 2004 Feb;38(3):733-9. doi: 10.1016/j.watres.2003.10.010.

DOI:10.1016/j.watres.2003.10.010

PMID:14723943

Abstract

The effects of chlorination on the elimination of three estrogenic chemicals such as 17beta-estradiol, nonylphenol and bis-phenol A were investigated using yeast two-hybrid assay (YTA), estrogen receptor (ER) competition assay (ER-CA), and high-performance liquid chromatography/mass spectrometry (LC/MS). The results of YTA, ER-CA and the analysis of LC/MS indicated that the estrogenic activity of the above-mentioned three endocrine disruptors were significantly reduced as a result of chlorination. The decrease in estrogenic activity paralleled a decrease in estrogenic chemicals under the influence of free chlorine. One common characteristic of estrogenic chemicals is the presence of a phenolic ring. Considering that a phenolic ring is likely to undergo some sort of transformation in an aqueous chlorination solution, the above-mentioned results may be applied to the rest of the estrogenic chemicals in natural waters.

摘要

采用酵母双杂交试验（YTA）、雌激素受体（ER）竞争试验（ER-CA）和高效液相色谱/质谱联用（LC/MS）研究了氯化作用对17β-雌二醇、壬基酚和双酚A这三种雌激素类化学物质去除效果的影响。YTA、ER-CA的结果以及LC/MS分析表明，上述三种内分泌干扰物的雌激素活性因氯化作用而显著降低。在游离氯的影响下，雌激素活性的降低与雌激素类化学物质的减少相平行。雌激素类化学物质的一个共同特征是存在酚环。考虑到酚环在氯化水溶液中可能会发生某种转化，上述结果可能适用于天然水体中其他的雌激素类化学物质。