Khemapech S, Monsiri K, Patumraj S, Siriviriyakul P
Interdepartment of Physiology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand.
Clin Hemorheol Microcirc. 2003;29(3-4):271-7.
The objective of this study is to examine the effects of genistein on endothelial dysfunction in bilateral ovariectomized rats. Female Wistar rats were subjected to a bilateral ovariectomy (OVX rat). The animals were divided into three groups: sham treated with vehicle (DMSO 100 microliters/day, Shamveh), OVX treated with vehicle (DMSO 100 microliters/day, OVXveh), and OVX treated with genistein (0.25 mg/kg BW/day, OVXgen). Mean arterial pressure (MAP), heart rate (HR), body weight (BW), uterine weight and plasma E2 were monitored at 4-week after the treatment. We investigated the endothelium-dependent and -independent vasorelaxation by using acetylcholine (Ach 10(-6) M) and sodium nitroprusside (SNP 10(-7) M), respectively. The experimental results indicated that the uterine weights of all OVX rats were significantly decreased as compared to the sham groups (OVXveh = 0.007+/-0.004 g, OVXgen =0.003+/-0.001 g, Shamveh =0.017+/-0.001 g). MAP of OVXveh group was significantly increased compared to the Sham group (OVXveh=139.99+/-7.50 mmHg, Shamveh =118.10+/-19.33 mmHg, p<0.05). No significant increase in MAP was observed in OVXgen (OVXgen =123.33+/-8.61 mmHg; p<0.05). HR showed no significant difference among those groups. The present study of vasodilator responses demonstrated only the significant decrease in endothelium-dependent, not for endothelium-independence, in OVX rats, while the treatment of genistein could significantly attenuate this abnormality (OVXveh =3.03+/-3.99%, Shamveh =45.46+/-3.59%, OVXgen =33.52+/-3.25% in % change of vessel diameter). The present findings suggest that genistein could be used as a therapeutic agent for menopausal vascular complications.
本研究的目的是检测染料木黄酮对双侧卵巢切除大鼠内皮功能障碍的影响。将雌性Wistar大鼠进行双侧卵巢切除术（OVX大鼠）。动物被分为三组：用溶剂处理的假手术组（二甲基亚砜100微升/天，假手术溶剂组）、用溶剂处理的OVX组（二甲基亚砜100微升/天，OVX溶剂组）和用染料木黄酮处理的OVX组（0.25毫克/千克体重/天，OVX染料木黄酮组）。在处理4周后监测平均动脉压（MAP）、心率（HR）、体重（BW）、子宫重量和血浆雌二醇（E2）。我们分别使用乙酰胆碱（10⁻⁶摩尔/升的Ach）和硝普钠（10⁻⁷摩尔/升的SNP）研究内皮依赖性和非内皮依赖性血管舒张。实验结果表明，与假手术组相比，所有OVX大鼠的子宫重量均显著降低（OVX溶剂组 = 0.007±0.004克，OVX染料木黄酮组 = 0.003±0.001克，假手术溶剂组 = 0.017±0.001克）。与假手术组相比，OVX溶剂组的MAP显著升高（OVX溶剂组 = 139.99±7.50毫米汞柱，假手术溶剂组 = 118.10±19.33毫米汞柱，p<0.05）。在OVX染料木黄酮组中未观察到MAP的显著升高（OVX染料木黄酮组 = 123.33±8.61毫米汞柱；p<0.05）。HR在这些组之间没有显著差异。本研究的血管舒张反应表明，OVX大鼠仅内皮依赖性血管舒张显著降低，而非内皮依赖性血管舒张未降低，而染料木黄酮处理可显著减轻这种异常（血管直径变化百分比中，OVX溶剂组 = 3.03±3.99%，假手术溶剂组 = 进行翻译时，请确保译文准确传达原文的含义，同时遵循中文的表达习惯。本研究的血管舒张反应表明，OVX大鼠仅内皮依赖性血管舒张显著降低，而非内皮依赖性血管舒张未降低，而染料木黄酮处理可显著减轻这种异常（血管直径变化百分比中，OVX溶剂组 = 3.03±3.99%，假手术溶剂组 = 45.46±3.59%，OVX染料木黄酮组 = 33.52±3.25%）。本研究结果表明，染料木黄酮可作为治疗绝经后血管并发症的药物。 45.46±3.59%，OVX染料木黄酮组 = 33.52±3.25%）。本研究结果表明，染料木黄酮可作为治疗绝经后血管并发症的药物。
