Tosukhowong P, Sangwatanaroj S, Jatuporn S, Prapunwattana P, Saengsiri A, Rattanapruks S, Srimahachota S, Udayachalerm W, Tangkijvanich P
Department of Biochemistry, King Chulalongkorn Memorial Hospital, Bangkok, Thailand.
Clin Hemorheol Microcirc. 2003;29(3-4):321-9.
An imbalance between oxidative damage and antioxidative protection in association with the pathophysiology of atherosclerosis has been suggested. The aim of our study was to investigate the relationship between plasma lipids, the antioxidant system and oxidative damage in Thai patients with stable coronary artery disease (CAD). Sixty-one patients (40 males, 21 females), who were angiographically defined as having CAD and were clinically stable, participated in this study. Thirty-two healthy subjects (20 males, 12 females) served as normal controls. The investigation included the measurements of plasma lipid profiles and plasma total antioxidative status (TAS) such as plasma vitamin E erythrocyte glutathione (GSH) and glutathione peroxidase (GPx), as well as malondialdehyde (MDA) and total plasma total protein thiols (P-SH). In patients with CAD, erythrocyte GSH and GPx were significantly lower than those found in controls. However plasma TAS and vitamin E were not significantly different between groups. Patients with CAD also had higher MDA and lower P-SH levels than the controls, which represents the oxidative damage products of lipid and proteins. Multiple regression analysis revealed negative correlations between GSH and cholesterol, GSH and low density lipoprotein (LDL), vitamin E and MDA, as well as P-SH and MDA. This study demonstrated the status of oxidative stress in patients with stable CAD. Since oxidative stress is the imbalance between the total oxidants and antioxidants in the body, any single oxidant/antioxidant parameter may not reflect the overall oxidative stress system. Thus, in patients with CAD, diets with various types of antioxidants may be more beneficial in increasing antioxidant activity than any particular antioxidant supplementation.
氧化损伤与抗氧化保护之间的失衡与动脉粥样硬化的病理生理学有关。我们研究的目的是调查泰国稳定型冠状动脉疾病（CAD）患者的血脂、抗氧化系统与氧化损伤之间的关系。61名经血管造影确诊患有CAD且临床症状稳定的患者（40名男性，21名女性）参与了本研究。32名健康受试者（20名男性，12名女性）作为正常对照。研究内容包括测量血脂谱、血浆总抗氧化状态（TAS），如血浆维生素E、红细胞谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx），以及丙二醛（MDA）和血浆总蛋白巯基（P-SH）。CAD患者的红细胞GSH和GPx显著低于对照组。然而，两组间血浆TAS和维生素E无显著差异。CAD患者的MDA水平也高于对照组，P-SH水平低于对照组，这代表了脂质和蛋白质的氧化损伤产物。多元回归分析显示，GSH与胆固醇、GSH与低密度脂蛋白（LDL）、维生素E与MDA以及P-SH与MDA之间呈负相关。本研究证实了稳定型CAD患者的氧化应激状态。由于氧化应激是体内总氧化剂和抗氧化剂之间的失衡，任何单一的氧化剂/抗氧化剂参数可能无法反映整体氧化应激系统。因此，对于CAD患者，摄入含有各种抗氧化剂的饮食可能比补充任何特定的抗氧化剂更有利于提高抗氧化活性。
