Xia Zuo-Li, Sun Bao-Liang, Yang Ming-Feng, Yuan Hui, Qiu Ping-Ming, Chen Yu-She
Institute of Microcirculation, Affiliated Hospital of Taishan Medical College, Taian, Shandone 271000, China.
Clin Hemorheol Microcirc. 2003;29(3-4):345-9.
Disputes on the significance of cerebral lymphatic drainage pathways under physiological and pathophysiological conditions still exist. The purpose of this experiment is to investigate the influence of cerebral lymphatic blockage on cerebral blood flow and cortex somatosensory evoked potential. Wistar rat cerebral lymphatic blockage models were established by removing cervical lymphatic nodes after obstructing their input and output tubes. Animals were divided randomly into a sham-operated group and a cerebral lymphatic-blockage group. Regional cerebral blood flow in different regions of the cortex were detected using a laser-Doppler flowmeter probe, and cortex evoked potential was detected using an electromyogram and evoked potential instrument before the operation, then 1 day, 5 days and 7 days after the operation. Results showed that the sham operation had no obvious effect on regional cerebral blood flow and the latency of somatosensory evoked potential. From 1 day to 7 days after cerebral lymphatic obstruction, regional cerebral blood flow in different cortical regions decreased markedly (P<0.01). Latency of somatosensory evoked potential was significantly delayed on the 5th and 7th day after blockage of cerebral lymphatic drainage (P<0.01). We concluded that cerebral lymphatic drainage may play an important role in maintaining the equilibrium of the internal environment of the brain, and blockage of this pathway results in cerebral ischemia.
关于脑淋巴引流途径在生理和病理生理条件下的意义仍存在争议。本实验的目的是研究脑淋巴阻塞对脑血流量和皮层体感诱发电位的影响。通过在阻塞颈淋巴结的输入和输出管后切除颈淋巴结，建立Wistar大鼠脑淋巴阻塞模型。将动物随机分为假手术组和脑淋巴阻塞组。在手术前、术后1天、5天和7天，使用激光多普勒流量计探头检测皮层不同区域的局部脑血流量，并使用肌电图和诱发电位仪检测皮层诱发电位。结果显示，假手术对局部脑血流量和体感诱发电位的潜伏期无明显影响。脑淋巴阻塞后1天至7天，不同皮层区域的局部脑血流量明显下降（P<0.01）。脑淋巴引流阻塞后第5天和第7天，体感诱发电位的潜伏期明显延迟（P<0.01）。我们得出结论，脑淋巴引流可能在维持脑内环境平衡中起重要作用，该途径的阻塞会导致脑缺血。
