Li Ailing, Li Hongwei, Zhang Jing, Jin Gang, Xiu Ruijuan
Institute of Microcirculation, Chinese Academy Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing 100005, China.
Clin Hemorheol Microcirc. 2003;29(3-4):375-82.
This study was designed to observe the effect of tumor conditioned medium (TCM) on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs). HUVECs were exposed to TCM from breast carcinoma cell line MDA-MB-231, then we measured their proliferation, apoptosis and cell cycle distribution by MTT and flow cytometery (FCM). Following the stimulation of TCM, HUVECs showed higher pro-mitogenic and anti-apoptotic ability than did the negative control group (ECGF-free medium with 20% FBS), but a similar ability to the positive control group (medium with ECGF and 20% FBS). From these results, we can conclude that breast carcinoma cell line MDA-MB-231 could secret soluble pro-angiogenic factors that induce HUVEC angiogenic switching, including cell cycle progression, proliferation and growth. The role and character of these factors remain to be further studied.
本研究旨在观察肿瘤条件培养基（TCM）对人脐静脉内皮细胞（HUVECs）增殖和凋亡的影响。将HUVECs暴露于乳腺癌细胞系MDA-MB-231的TCM中，然后通过MTT和流式细胞术（FCM）检测其增殖、凋亡及细胞周期分布。经TCM刺激后，HUVECs显示出比阴性对照组（不含内皮细胞生长因子且含20%胎牛血清的培养基）更高的促有丝分裂和抗凋亡能力，但与阳性对照组（含内皮细胞生长因子和20%胎牛血清的培养基）能力相似。从这些结果中，我们可以得出结论，乳腺癌细胞系MDA-MB-231能够分泌可溶性促血管生成因子，诱导HUVECs发生血管生成转换，包括细胞周期进程、增殖和生长。这些因子的作用和特性仍有待进一步研究。
