Cayé-Thomasen Per, Tos Mirko
Department of Oto-Rhino-Laryngology, Head and Neck Surgery, Gentofte University Hospital of Copenhagen, Hellerup, Denmark.
Otol Neurotol. 2004 Jan;25(1):14-8. doi: 10.1097/00129492-200401000-00003.
To investigate the histomorphologic impact of acute otitis media on the subepithelial glands of the eustachian tube. Previous investigations have shown an increase of eustachian tube goblet cell density during and up to at least 6 months after acute otitis media.
Longitudinal study in an experimental animal model of acute otitis media caused by Streptococcus pneumoniae.
Bacterial middle ear challenge, after which the animals were killed, in groups of five rats on Days 4, 8, 16, 90, and 180. Dissection and preparation of the eustachian tube and adjacent structures, followed by serial transverse sectioning, periodic acid-Schiff/alcian blue staining, and qualitative/quantitative morphometric light-microscopic investigations of the histomorphology of the eustachian tube glands, in particular, the stainability, composition, volume, and number of gland acini.
The volume of the eustachian tube glands progressed to peak 16 days after inoculation, followed by a gradual normalization. The volume was still increased 3 months after the acute infection but completely normalized after 6 months. The increase was primarily due to hypertrophy of the mucous gland components, as the serous gland component volume and number of gland acini was unchanged. The periodic acid-Schiff/alcian blue staining of the mucous gland acini changed temporarily during the acute infection, indicating changes in mucus glycoprotein composition, from neutral to acidic/sulfated.
The volume of the eustachian tube glands increases during and up to at least 3 months after acute otitis media, primarily because of hypertrophy of the mucous gland components. This may compromise tubal ventilatory and drainage function.
研究急性中耳炎对咽鼓管上皮下腺体的组织形态学影响。既往研究表明，在急性中耳炎期间及之后至少6个月内，咽鼓管杯状细胞密度会增加。
在肺炎链球菌引起的急性中耳炎实验动物模型中进行纵向研究。
对动物进行中耳细菌攻击，之后在第4、8、16、90和180天，将大鼠按每组5只处死。解剖并制备咽鼓管及相邻结构，随后进行连续横切、过碘酸-希夫/阿尔辛蓝染色，以及对咽鼓管腺体组织形态进行定性/定量形态学光学显微镜检查，特别是腺泡的染色性、组成、体积和数量。
咽鼓管腺体体积在接种后16天达到峰值，随后逐渐恢复正常。急性感染后3个月时体积仍增加，但6个月后完全恢复正常。增加主要是由于黏液腺成分肥大，浆液腺成分体积和腺泡数量未变。急性感染期间，黏液性腺泡的过碘酸-希夫/阿尔辛蓝染色暂时改变，表明黏液糖蛋白组成从中性变为酸性/硫酸化。
急性中耳炎期间及之后至少3个月内，咽鼓管腺体体积增加，主要是由于黏液腺成分肥大。这可能会损害咽鼓管的通气和引流功能。
