一种从酿酒酵母固醇突变体中分离酵母甾醇的简单方法。
A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae.
作者信息
Heiderpriem R W, Livant P D, Parish E J, Barbuch R J, Broaddus M G, Bard M
机构信息
Department of Chemistry, Auburn University, AL 36849.
出版信息
J Steroid Biochem Mol Biol. 1992 Dec;43(7):741-3. doi: 10.1016/0960-0760(92)90300-8.
PMID:1472465
Abstract
A simple method is described for the direct isolation of zymosterol (5 alpha-cholesta-8,24-dien-3 beta-ol) of high purity from a sterol mutant of Saccharomyces cerevisiae. This yeast strain, which is a double mutant of the ERG6 (sterol transmethylase) and ERG2 (C-8 sterol isomerase) genes, accumulates zymosterol as its major sterol component.
摘要
描述了一种从酿酒酵母的甾醇突变体中直接分离高纯度酵母甾醇（5α-胆甾-8,24-二烯-3β-醇）的简单方法。该酵母菌株是ERG6（甾醇转甲基酶）和ERG2（C-8甾醇异构酶）基因的双突变体，以酵母甾醇作为其主要甾醇成分进行积累。
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