Zhang Song, Wang En Zhong, Bai Chun Xue, Xu Yong Hua
Zhong San Hospital, Fu Dan University, Shanghai 200032, China.
Shi Yan Sheng Wu Xue Bao. 2003 Oct;36(5):375-80.
To observe the changes of phenotype, proliferation activity and cytotoxicity of CIK(cytokine induced killer) cells after co-culturing with dendritic cells(DCs), DCs and CIK cells were generated, respectively, by cytokines induction of culturing PBMC of healthy blood donor. The typical DCs and DCs pulsed by A549 lung cancer cells lysate antigen were co-cultured with CIK cells, respectively. Cell surface markers were analyzed by FACS method. IFN-gamma and IL-12 secreted by CIK cells and co-cultured cells were detected by ELISA. The cytotoxicities of effective cells on A549 cells and BEL-7404 cells in vitro were measured by MTT assays. The results showed that co-culture of DCs with CIK cells produced a new cell population, whose proliferation activity and cytotoxicity were much higher than CIK cells. The co-culture stimulated the maturation of DCs. The co-culture of CIK cells and A549 cells lysate antigen pulsed DC resulted in an enhanced killing activity to A549 cells than CIK cells and un-pulsed DC-CIK cells(p < 0.05). In conclusion, CIK cells co-cultured with DCs are more powerful than CIK cells alone in anti-tumor reaction.
为观察细胞因子诱导的杀伤细胞（CIK）与树突状细胞（DC）共培养后CIK细胞的表型、增殖活性及细胞毒性变化，分别通过细胞因子诱导培养健康献血者外周血单个核细胞（PBMC）来生成DC和CIK细胞。将典型的DC以及经A549肺癌细胞裂解物抗原脉冲处理的DC分别与CIK细胞共培养。采用荧光激活细胞分选（FACS）法分析细胞表面标志物。通过酶联免疫吸附测定（ELISA）检测CIK细胞及共培养细胞分泌的干扰素-γ（IFN-γ）和白细胞介素-12（IL-12）。采用噻唑蓝（MTT）比色法测定效应细胞对体外A549细胞和BEL-7404细胞的细胞毒性。结果显示，DC与CIK细胞共培养产生了一个新的细胞群体，其增殖活性和细胞毒性远高于CIK细胞。共培养促进了DC的成熟。CIK细胞与经A549细胞裂解物抗原脉冲处理的DC共培养后，对A549细胞的杀伤活性比CIK细胞及未脉冲处理的DC-CIK细胞增强（p<0.05）。综上所述，CIK细胞与DC共培养在抗肿瘤反应中比单独的CIK细胞更具优势。
