Cucuianu M, Trif I
Department of Clinical Biochemistry, University of Cluj Medical School.
Rev Roum Physiol (1990). 1992 Jan-Jun;29(1-2):33-8.
After briefly reviewing the literature concerning the role of leukocytes and platelets in coagulation and fibrinolysis, the authors present their own results on the effect of intact platelets and of platelet releasate on tissue plasminogen activator-induced lysis of plasma clots. At a final concentration of 7 IU/ml of tissue plasminogen activator in the clotted mixture, a suspension of intact platelets (110 x 10(6)/ml in final concentration) produced an acceleration of clot lysis, while the thrombin-induced platelet releasate obtained from the same platelet suspension caused an obvious inhibition of fibrinolysis. The respective mean lysis times obtained in 7 experiments were 91 min +/- 7.76 (mean +/- SEM) for the control clots, 65 min +/- 5.8 for the clots containing platelets and 114 min +/- 11.5 for the clots including platelet releasate. The statistical significance versus controls, calculated by paired difference analysis was p < 0.002 and p < 0.001, respectively. The results suggest that in the context of a potent activation of fibrinolysis the platelet surface would enhance the process by favouring the interaction between plasminogen and its activator, while the platelet releasate rich inhibitors would increase the resistance to lysis of the plasma clot.
在简要回顾了有关白细胞和血小板在凝血和纤维蛋白溶解中作用的文献后，作者展示了他们自己关于完整血小板和血小板释放物对组织纤溶酶原激活物诱导的血浆凝块溶解作用的研究结果。在凝血混合物中组织纤溶酶原激活物的终浓度为7 IU/ml时，完整血小板悬液（终浓度为110×10⁶/ml）可加速凝块溶解，而从相同血小板悬液中获得的凝血酶诱导的血小板释放物则明显抑制纤维蛋白溶解。在7次实验中，对照凝块的平均溶解时间为91分钟±7.76（平均值±标准误），含血小板凝块的平均溶解时间为65分钟±5.8，含血小板释放物凝块的平均溶解时间为114分钟±11.5。通过配对差异分析计算得出，与对照相比的统计学显著性分别为p<0.002和p<0.001。结果表明，在纤维蛋白溶解强力激活的情况下，血小板表面会通过促进纤溶酶原与其激活物之间的相互作用来增强这一过程，而富含抑制剂的血小板释放物会增加血浆凝块对溶解的抵抗力。
