Johnson M P, Wooding F B
Histochem J. 1978 Mar;10(2):171-83. doi: 10.1007/BF01003302.
Lactating mammary tissue from farm animals and small mammals was perfusion-fixed, prior to histochemical procedures, in an effort to localize the ouabain-sensitive Na+/K+-stimulated ATPase enzyme with the use of specific inhibitors. Histochemical evidence suggests that the Na+/K+-stimulated ATPase is located predominantly on the cytoplasmic side of the plasmalemma in myoepithelial and secretory cells of the alveolus and in epithelial cells of the ducts. Enzyme is not present on the apical plasmalemma of the secretory or duct cells. This substantiates the previous suggestions of localization of the sodium pumps on lateral and basal surface in lactating mammary secretory cells and duct cells.
在进行组织化学程序之前，对来自农场动物和小型哺乳动物的泌乳乳腺组织进行灌注固定，以便使用特定抑制剂定位哇巴因敏感的钠钾激活ATP酶。组织化学证据表明，钠钾激活ATP酶主要位于肺泡肌上皮细胞和分泌细胞以及导管上皮细胞的质膜细胞质侧。分泌细胞或导管细胞的顶端质膜上不存在该酶。这证实了之前关于泌乳乳腺分泌细胞和导管细胞中钠泵定位于侧面和基底表面的观点。
