Netto Cristina B, Siqueira Ionara R, Fochesatto Cíntia, Portela Luis V, da Purificação Tavares Maria, Souza Diogo O, Giugliani Roberto, Gonçalves Carlos Alberto
Departamento de Bioquímica, ICBS, UFRGS, Porto Alegre RS, Brazil.
Clin Biochem. 2004 Feb;37(2):134-7. doi: 10.1016/j.clinbiochem.2003.09.010.
We measured S100B levels and superoxide dismutase (SOD) activity retrospectively in amniotic fluid samples from pregnancies with normal and Down syndrome (DS) fetuses.
Samples from 26 normal and 71 Down syndrome fetuses were studied. S100B protein levels were determined using LIA-mat Sangtec kit, and SOD activity was measured with the RANSOD kit.
We observed significantly higher levels of S100B in the Down group (median of 1.24 microg/l) than in the control group (median 0.69 microg/l). S100B concentration in DS samples increased from the 13th to the 18th week of gestation and was positively correlated with gestational age. The amniotic fluid SOD activity in the DS group (16.60 U/mg/prot) was significantly higher than in the normal one (10.78 U/mg/prot).
This study indicates that S100B and SOD in amniotic fluid could be used as additional parameters for prenatal screening of trissomy 21 and that S100B values are associated with the gestational age.
我们回顾性测量了正常胎儿和唐氏综合征（DS）胎儿妊娠的羊水样本中的S100B水平和超氧化物歧化酶（SOD）活性。
研究了26例正常胎儿和71例唐氏综合征胎儿的样本。使用LIA - mat Sangtec试剂盒测定S100B蛋白水平，并用RANSOD试剂盒测量SOD活性。
我们观察到唐氏综合征组的S100B水平（中位数为1.24微克/升）显著高于对照组（中位数为0.69微克/升）。唐氏综合征样本中的S100B浓度从妊娠第13周增加到第18周，且与孕周呈正相关。唐氏综合征组的羊水SOD活性（16.60 U/mg/蛋白）显著高于正常组（10.78 U/mg/蛋白）。
本研究表明羊水S100B和SOD可作为21三体产前筛查的附加参数，且S100B值与孕周相关。
