Asakura Hiroshi, Kawamoto Keiko, Shirahata Toshikazu, Makino Sou-ichi
Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, 080-8555, Hokkaido, Japan
Microb Pathog. 2004 Mar;36(3):147-51. doi: 10.1016/j.micpath.2003.10.006.
The NaCl sensitivity of Salmonella enterica serovar Oranienburg strains depends on their origin. We found previously that food- and patient-origin isolates in an outbreak were, respectively, NaCl-resistant and NaCl-sensitive, and the NaCl-resistant strain of food-origin isolates became NaCl-sensitive after passage of the strain through mice [FEMS Microbiol. Lett. 212 (2002) 87]. Here, we report that this phenotypic difference is mainly dependent on topological changes regulated by H-NS, a bacterial histone-like nucleoid protein that binds non-specifically to DNA. That is, this phenotypic difference was caused by changes in DNA topology during infection of the host. Based on these findings, we propose this mechanism has a key role in promoting the survival of Salmonella under osmotic stress.
肠炎沙门氏菌血清型奥拉宁堡菌株对氯化钠的敏感性取决于其来源。我们之前发现，一次疫情中来自食品和患者的分离株分别对氯化钠具有抗性和敏感性，并且食品来源分离株中的耐氯化钠菌株在通过小鼠传代后变得对氯化钠敏感[《FEMS微生物学快报》212 (2002) 87]。在此，我们报告这种表型差异主要取决于由H-NS调节的拓扑变化，H-NS是一种与DNA非特异性结合的细菌类组蛋白核仁蛋白。也就是说，这种表型差异是由宿主感染期间DNA拓扑结构的变化引起的。基于这些发现，我们提出这种机制在促进沙门氏菌在渗透胁迫下的存活中起关键作用。
