Zhang Ning, Suh Sung-Oui, Blackwell Meredith
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
J Invertebr Pathol. 2003 Nov;84(3):226-33. doi: 10.1016/j.jip.2003.10.002.
We have regularly cultured yeasts from the gut of certain beetles in our ongoing research. In this study cloned PCR products amplified from the gut contents of certain mushroom-feeding and wood-ingesting beetles in four families (Erotylidae, Tenebrionidae, Ciidae, and Passalidae) were sequenced and compared with culture results. Cultural techniques detected some yeasts present in the gut of the beetles, including a Pichia stipitis-like yeast associated with wood-ingesting passalid beetles. Clone sequences similar to several ascomycete yeasts and Malassezia restricta, a fastidious basidiomycetous yeast requiring special growth media, however, were not detected by culturing. Unexpectedly, phylogenetic analysis of additional clone sequences discovered from passalid beetles showed similarity to members of the Parabasalia, protists known from other wood-ingesting insects, termites, and wood roaches. Examination of all gut regions of living passalids, however, failed to reveal parabasalids, and it is possible that they were parasites in the gut tissue present in low numbers.
在我们正在进行的研究中，我们定期从某些甲虫的肠道中培养酵母。在这项研究中，对从四个科（拟步甲科、拟步甲科、谷盗科和扁甲科）的某些以蘑菇为食和以木材为食的甲虫肠道内容物中扩增得到的克隆PCR产物进行了测序，并与培养结果进行了比较。培养技术检测到了甲虫肠道中存在的一些酵母，包括一种与以木材为食的扁甲甲虫相关的树干毕赤酵母样酵母。然而，通过培养未检测到与几种子囊菌酵母和限制马拉色菌（一种需要特殊生长培养基的挑剔担子菌酵母）相似的克隆序列。出乎意料的是，从扁甲甲虫中发现的其他克隆序列的系统发育分析表明，它们与其他以木材为食的昆虫、白蚁和木蟑螂中已知的原生生物披发虫纲成员相似。然而，对活的扁甲甲虫所有肠道区域的检查未能发现披发虫，它们有可能是存在于肠道组织中数量较少的寄生虫。
