Dalziel R G, Mendelson S C, Quinn J P
Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, Summerhall, Edinburgh.
Autoimmunity. 1992;13(4):265-7. doi: 10.3109/08916939209112334.
Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.
针对从T细胞系MLA144制备的核提取物中纯化的Ku复合物的85 kDa和70 kDa单个亚基制备了多克隆抗体。通过蛋白质免疫印迹分析,它们能特异性识别HeLa细胞全细胞提取物中Ku复合物的相应亚基。利用荧光激活细胞分选分析,它们还能够鉴定细胞膜中存在的Ku蛋白。