Suppr超能文献

核自身免疫抗原Ku也存在于细胞表面。

The nuclear autoimmune antigen Ku is also present on the cell surface.

作者信息

Dalziel R G, Mendelson S C, Quinn J P

机构信息

Department of Veterinary Pathology, Royal (Dick) School of Veterinary Studies, Summerhall, Edinburgh.

出版信息

Autoimmunity. 1992;13(4):265-7. doi: 10.3109/08916939209112334.

Abstract

Polyclonal antibodies were raised against the individual 85 and 70 kDa subunits of the Ku complex purified from nuclear extract prepared from the T cell line MLA144. They specifically recognise the appropriate subunits of the Ku complex from whole cell extract of HeLa cells using Western blot analysis. They are also able to identify the Ku proteins present in the cell membrane using FACS analysis.

摘要

针对从T细胞系MLA144制备的核提取物中纯化的Ku复合物的85 kDa和70 kDa单个亚基制备了多克隆抗体。通过蛋白质免疫印迹分析,它们能特异性识别HeLa细胞全细胞提取物中Ku复合物的相应亚基。利用荧光激活细胞分选分析,它们还能够鉴定细胞膜中存在的Ku蛋白。

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验